最新中文字幕-精品国产99-国产精品福利在线观看-粉嫩av一区二区三区-日韩精品久久久久-美丽的姑娘国语视频-亚洲欧洲色-国产不卡毛片-三级黄毛片-免费福利在线-日韩av三区-欧美成年人网站-欧洲美女与动交ccoo-成人涩涩软件-秋霞影院午夜老牛影院-婷婷久久伊人-呦呦视频在线观看-www.天天综合-日韩精品高清视频-国产欧美综合在线-欧美日韩一区二区三区在线视频-a猛片免费播放-91九色蝌蚪国产-国产欧美日韩专区-嫩模被强到高潮呻吟不断

歷史搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > NCI-H510A [H510A, NCI-H510] B165348
NCI-H510A [H510A, NCI-H510]
NCI-H510A [H510A, NCI-H510]
規格:
價格:
編號:B165348
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
NCI-H510A [H510A, NCI-H510]
商品貨號
B165348
Organism
Homo sapiens, human
Tissue
lung; derived from metastatic tissue: adrenal gland
培養基
Product Format
frozen
Morphology
epithelial
Culture Properties
mixed, adherent and suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
carcinoma; small cell lung cancer; extrapulmonary origin
Age
56 years
Gender
male
Ethnicity
Caucasian
Storage Conditions
liquid nitrogen vapor phase
Karyotype
hypotriploid; modal number = 54; range = 46 to 57. Twenty to 25 marker chromosomes were common to all cells. These included t(13q21q), der(1)t(1;21)(p36.1;q11), der(7)t(7;7)(p22;q22), 11p+, 12p+, and 15p+. Neither DM nor HSR were detected; structurally normal N1, N2, N13 and N21 were absent. Generally, there were 3 copies of both F group chromosomes; the X chromosomes were paired, and structurally normal Y chromosome was not found.
Images
Derivation
The NCI-H510A cell line was derived by D. Carney, A.F. Gazdar and associates in 1982 from an adrenal metastasis in an adult male patient.
Clinical Data
56 years
Caucasian
male
Tumorigenic
Yes
Effects
Yes, in nude mice
Comments

The cells produce easily detectable p53 mRNA at levels comparable to those in normal lung tissue. 

The line does not exhibit any gross structural DNA abnormalities. 

The cells express elevated levels of four biochemical markers of SCLC: neuron specific enolase, the brain isoenzyme of creatine kinase, L-DOPA decarboxylase and bombesin-like immunoreactivity.

The cells form transplantable tumors with typical small cell carcinoma histology.

Complete Growth Medium
The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
This line grows as a mixture of cells in suspension and adherent cells. Subcultures can be prepared by scraping the adherent cells into the medium, collecting the cells by centrifugation, resuspending in fresh medium and dispensing into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: culture medium, 92.5%; DMSO, 7.5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 10,12
D13S317: 11,12
D16S539: 13
D5S818: 9,12
THO1: 7,9.3
TPOX: 8
vWA: 15
Name of Depositor
AF Gazdar, JD Minna
Deposited As
Homo sapiens
Year of Origin
1982
References

Takahashi T, et al. p53: A frequent target for genetic abnormalities in lung cancer. Science 246: 491-494, 1989. PubMed: 2554494

Carney DN, et al. Establishment and identification of small cell lung cancer cell lines having classic and variant features. Cancer Res. 45: 2913-2923, 1985. PubMed: 2985257

  • 王經理
    手機號:17280875617
    QQ:1438578920
  • 胡經理
    手機號:13345964880
    QQ:2438244627
  • 周經理
    手機號:17757487661
    QQ:1296385441
  • 于經理
    手機號:18067160830
    QQ:2088210172
  • 沈經理
    手機號:19548299266
    QQ:2662369050
  • 李經理
    手機號:13626845108
    QQ:972239479