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NCI-H520 [H520]

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價格:

編號:B165349

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

NCI-H520 [H520]

商品貨號

B165349

Organism

Homo sapiens, human

Tissue

lung

培養基

Product Format

frozen

Morphology

epithelial

Culture Properties

adherent

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

squamous cell carcinoma

Gender

male

Applications

This cell line is a suitable transfection host.

Storage Conditions

liquid nitrogen vapor temperature

Karyotype

This is a hypotriploid human cell line. The modal chromosome number is 58 occurring at 30%. The frequency of higher ploidies was 3.2%. Over 30 marker chromosomes were common to all cells, and four others were found in some cells. Among the common markers were 1q+, t(1q8q), 2q+, der(16)t(3;16)(q21;q22), der(19)t(13;19)(q21;q13), and der(5)t(5;5)(p15pq13). Generally, there were two copies of der(5) in each cell. Normal Y and D group chromosomes were absent, and the X chromosome was single.

Derivation

The NCI-H520 cell line was derived by A.F. Gazdar and associates in 1982 from a sample of a lung mass taken from a patient with squamous cell carcimoma of the lung.

Clinical Data

male

Tumorigenic

Yes

Effects

Yes, in nude mice inoculated subcutaneously with 10(7) cells

(Tumors developed within 21 days at 100% frequency (5/5).)

Comments

The cells express greatly reduced levels of p53 mRNA relative to normal lung tissue, but exhibit no gross structural DNA abnormalities.

The cells stain positively for keratin and vimentin but are negative for neurofilament triplet protein.

The line can be cloned in soft agar (with or without serum).

Complete Growth Medium

The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.

Subculturing

Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.

Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended

Medium Renewal: 2 to 3 times per week

Cryopreservation

Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor temperature

Culture Conditions

Temperature: 37°C

STR Profile

Amelogenin: X

CSF1PO: 10

D13S317: 10,11

D16S539: 8,13

D5S818: 12,13

D7S820: 8,12

THO1: 10

TPOX: 8

vWA: 18,19

Isoenzymes

AK-1, 1

ES-D, 1

G6PD, B

GLO-I, 2

Me-2, 0

PGM1, 1

PGM3, 1

Population Doubling Time

61 hrs in medium with serum; 25 to 32 hrs in serum-free medium

Name of Depositor

AF Gazdar, JD Minna

Deposited As

Homo sapiens

References

Banks-Schlegel SP, et al. Intermediate filament and cross-linked envelope expression in human lung tumor cell lines. Cancer Res. 45: 1187-1197, 1985. PubMed: 2578876

Takahashi T, et al. p53: A frequent target for genetic abnormalities in lung cancer. Science 246: 491-494, 1989. PubMed: 2554494

Brower M, et al. Growth of cell lines and clinical specimens of human non-small cell lung cancer in a serum-free defined medium. Cancer Res. 46: 798-806, 1986. PubMed: 3940644

Tumors developed within 21 days at 100% frequency (5/5).

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