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NCI-H596 [H596]

規格:

價格:

編號:B165353

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

NCI-H596 [H596]

商品貨號

B165353

Organism

Homo sapiens, human

Tissue

lung

培養基

Product Format

frozen

Morphology

epithelial

Culture Properties

adherent

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

adenosquamous carcinoma

Age

73 years

Gender

male

Ethnicity

Caucasian

Applications

This cell line is a suitable transfection host.

Storage Conditions

liquid nitrogen vapor phase

Karyotype

modal number = 71; range = 65 to 75.
This is a near triploid human cell line. The modal chromosome number was 71 although cells with 70 chromosomes occurred frequently. The frequency of higher ploidies was 2.9%.; Over 11 marker chromosomes were common to all cells, and at least 6 others were found in some cells. Among the common markers were: del(1)(p22), i(3q), i(6p), i(7p), t(7p17q), 14q+ and five others.; Of these, del(1) and i(7p) were generally double [occasionally triple for del(1)]. Constantly, there were 2 to 3 minute chromosomes that were about 1/2 the size of a G group chromosome.; Structurally normal N17 had only a single copy per cell, whereas N15 had four copies and N20 and N21 had four or more copies. The X chromosome was paired, but the Y was not found by fluorescence microscopy.

Images

Derivation

The NCI-H596 cell line was derived by A.F. Gazdar and H. Oie in 1983 from a tumor mass in the chest wall of a patient with adenosquamous carcinoma of the lung.

The specimen was obtained prior to therapy.

Clinical Data

73 years

Caucasian

male

The NCI-H596 cell line was derived by A.F. Gazdar and H. Oie in 1983 from a tumor mass in the chest wall of a patient with adenosquamous carcinoma of the lung.

Tumorigenic

Yes

Effects

Yes, in nude mice

Comments

The cells express easily detectable levels of p53 mRNA (comparable to levels found in normal lung), and exhibit no gross structural DNA abnormalities.

The cells stain positively for keratin and vimentin, but are negative for neurofilament triplet protein.

The cells express multiple markers of squamous differentiation, including cross linked envelope formation, transglutaminase activity and production of involucrin.

Complete Growth Medium

The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.

Subculturing

Volumes are given for a 75 cm² flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.

Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.

Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended

Medium Renewal: 2 to 3 times per week

Cryopreservation

Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Temperature: 37°C

STR Profile

Amelogenin: X

CSF1PO: 12,13

D13S317: 11

D16S539: 11

D5S818: 11,13

D7S820: 11

THO1: 7,9.3

TPOX: 8

vWA: 16

Isoenzymes

AK-1, 1

ES-D, 1

G6PD, B

GLO-I, 1

Me-2, 0

PGM1, 1

PGM3, 1

Name of Depositor

AF Gazdar, JD Minna

Deposited As

Homo sapiens

Year of Origin

1983

References

Banks-Schlegel SP, et al. Intermediate filament and cross-linked envelope expression in human lung tumor cell lines. Cancer Res. 45: 1187-1197, 1985. PubMed: 2578876

Takahashi T, et al. p53: A frequent target for genetic abnormalities in lung cancer. Science 246: 491-494, 1989. PubMed: 2554494

Levitt ML, et al. Cross-linked envelope-related markers for squamous differentiation in human lung cancer cell lines. Cancer Res. 50: 120-128, 1990. PubMed: 1967140

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