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NCI-H660
NCI-H660
規格:
價格:
編號:B165356
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
NCI-H660
商品貨號
B165356
Organism
Homo sapiens, human
Tissue
prostate; derived from the metastatic site: lymph node
培養基
Cell Type
epithelial neuroendocrine
Product Format
frozen
Morphology
epithelial
Culture Properties
floating and attached clusters
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
stage E, small cell carcinoma
Age
63 years adult
Gender
male
Ethnicity
Caucasian
Storage Conditions
liquid nitrogen vapor phase
Derivation
Derived from metastatic site, lymph node
Clinical Data
63 years adult
Caucasian
male
Receptor Expression
atrial natriuretic peptide (ANP), expressed
HeLa Markers
N
Comments
While NCI-H660 has the appearance and many of the properties of small cell carcinoma, there are also differences. NCI-H660 expresses elevated levels of L-dopa carboxylase and bombesin-like immunoreactivity. The cells express functional atrial natriuretic peptide (ANP) receptors, but ANP addition produces no detectable change in the cells' growth pattern.
Complete Growth Medium
HITES medium supplemented with 5% fetal bovine serum
    The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No.30-2001. To make the complete growth medium, add the following components to the base medium
  1. 0.005 mg/ml Insulin
  2. 0.01 mg/ml Transferrin
  3. 30nM Sodium selenite (final conc.)
  4. 10 nM Hydrocortisone (final conc.)
  5. 10 nM beta-estradiol (final conc.)
  6. extra 2mM L-glutamine (for final conc. of 4 mM)
  7. 5% fetal bovine serum (final conc.)

Subculturing
Add medium as the cell density increases. Shake flask to dislodge attached cells. Single cells are often dead and clusters are viable. Subculture when there are numerous, healthy-appearing clusters present in suspension.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium, 40%;  fetal bovine serum, 50%; DMSO, 10%.
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 12
D13S317: 8
D16S539: 11
D5S818: 11
D7S820: 8,10
THO1: 7,9.3
TPOX: 8
vWA: 17
Name of Depositor
AF Gazdar, JD Minna
Deposited As
Homo sapiens
References

Lai SL, et al. Molecular genetic characterization of neuroendocrine lung cancer cell lines. Anticancer Res. 15: 225-232, 1995. PubMed: 7762988

Carney DN, et al. Establishment and identification of small cell lung cancer cell lines having classic and variant features. Cancer Res. 45: 2913-2923, 1985. PubMed: 2985257

Johnson BE, et al. Retention of chromosome 3 in extrapulmonary small cell cancer shown by molecular and cytogenetic studies. J. Natl. Cancer Inst. 81: 1223-1228, 1989. PubMed: 2569043

NCI-H660 was originally described as a small cell lung carcinoma [PubMed: 2985257], subsequently it was correctly identified as an extrapulmonary small cell carcinoma originating in the prostate gland [PubMed: 2569043, 7762988]. The line was derived by A. F. Gazdar, H. K. Oie, J. D. Minna and associates from a lymph node metastasis taken from a patient prior to therapy.

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