最新中文字幕-精品国产99-国产精品福利在线观看-粉嫩av一区二区三区-日韩精品久久久久-美丽的姑娘国语视频-亚洲欧洲色-国产不卡毛片-三级黄毛片-免费福利在线-日韩av三区-欧美成年人网站-欧洲美女与动交ccoo-成人涩涩软件-秋霞影院午夜老牛影院-婷婷久久伊人-呦呦视频在线观看-www.天天综合-日韩精品高清视频-国产欧美综合在线-欧美日韩一区二区三区在线视频-a猛片免费播放-91九色蝌蚪国产-国产欧美日韩专区-嫩模被强到高潮呻吟不断

歷史搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > NCI-H676B [H676B] B165358
NCI-H676B [H676B]
NCI-H676B [H676B]
規格:
價格:
編號:B165358
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
NCI-H676B [H676B]
商品貨號
B165358
Organism
Homo sapiens, human
Tissue
lung; Derived from metastatic site: pleural effusion
培養基
Product Format
frozen
Morphology
epithelial
Culture Properties
suspension, multicell aggregates (gland-like clusters in suspension; the viability of cells in clusters is better than the viability of the single cells in the cultures)
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
adenocarcinoma
Age
63 years
Gender
male
Ethnicity
Caucasian
Applications
The NCI-H676B cell line was derived by A.F. Gazdar and H. Oie in 1984 from the pleural fluid of a patient with adenocarcinoma of the lung taken prior to treatment.
The cells produce a abnormal size p53 mRNA (2.3 kb) as well as the normal size mRNA (2.8 kb).
The line does not exhibit any gross structural DNA abnormalities.
The cells produce mucin, and occasionally signet ring cells are formed.
Storage Conditions
liquid nitrogen vapor phase
Karyotype
This is a hypodiploid cell line; modal number = 43; range = 39 to 47. Marker chromosomes consisted of over 70% of the chromosomes in each cell complement. Among the identifiable markers were t(1q8q), der(3)t(3;?)(q29;?), t(2q7q), der(9)t(9;14)(p13;q11) and der(11)t(?q23;?). All markers had a single copy per cells. The N5, N9, N10, N17, N18, N20, N21 and the X were the only structurally normal chromosomes found. Except for the paired N5, all others had single copies per cell.
Derivation
The NCI-H676B cell line was derived by A.F. Gazdar and H. Oie in 1984 from the pleural fluid of a patient with adenocarcinoma of the lung taken prior to treatment.
Clinical Data
The NCI-H676B cell line was derived by A.F. Gazdar and H. Oie in 1984 from the pleural fluid of a patient with adenocarcinoma of the lung taken prior to treatment.
male
Caucasian
63 years
Genes Expressed
mucin
Cellular Products
mucin
Comments
The NCI-H676B cell line was derived by A.F. Gazdar and H. Oie in 1984 from the pleural fluid of a patient with adenocarcinoma of the lung taken prior to treatment.
The cells produce a abnormal size p53 mRNA (2.3 kb) as well as the normal size mRNA (2.8 kb).
The line does not exhibit any gross structural DNA abnormalities.
The cells produce mucin, and occasionally signet ring cells are formed.
Complete Growth Medium
ACL-4 medium (serum-free)
The base medium for this cell line is ATCC formulated DMEM: F12 Medium Catalog No. 30-2006. To make the complete growth medium, add the following components to the base medium:
  • 0.02 mg/ml insulin
  • 0.01 mg/ml transferrin
  • 25 nM sodium selenite (final conc.)
  • 50 nM Hydrocortisone (final conc.)
  • 1 ng/ml Epidermal Growth Factor (do not filter)
  • 0.01 mM ethanolamine (final conc.)
  • 0.01 mM phosphorylethanolamine (final conc.)
  • 100 pM triiodothyronine (final conc.)
  • 0.5% (w/v) bovine serum albumin (final conc.)
  • 10 mM HEPES
  • 0.5 mM sodium pyruvate (final conc.)
  • extra 2mM L-glutamine (for final conc. of 4.5mM)

Subculturing
Protocol: This line grows as aggregates of cells in suspension. Culture can be maintained by addition of medium or by replacement of medium. Alternatively, the cells may be collected by centrifugation and dispersed into fresh medium.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: culture medium, 82.5%; fetal bovine serum, 10%; DMSO, 7.5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
STR Profile
Amelogenin: X
CSF1PO: 9
D13S317: 10
D16S539: 11,12
D5S818: 11
D7S820: 12
THO1: 9.3
TPOX: 8,11
vWA: 16,19
Isoenzymes
AK-1, 2
ES-D, 1
G6PD, B
GLO-I, 1-2
Me-2, 0
PGM1, 1-2
PGM3, 2
Name of Depositor
AF Gazdar, JD Minna
Deposited As
Homo sapiens
Year of Origin
1984
References

Takahashi T, et al. p53: A frequent target for genetic abnormalities in lung cancer. Science 246: 491-494, 1989. PubMed: 2554494

gland-like clusters in suspension; the viability of cells in clusters is better than the viability of the single cells in the cultures

gland-like clusters in suspension; the viability of cells in clusters is better than the viability of the single cells in the cultures

  • 王經理
    手機號：17280875617
    QQ：1438578920
  • 胡經理
    手機號：13345964880
    QQ：2438244627
  • 周經理
    手機號：17757487661
    QQ：1296385441
  • 于經理
    手機號：18067160830
    QQ：2088210172
  • 沈經理
    手機號：19548299266
    QQ：2662369050
  • 李經理
    手機號：13626845108
    QQ：972239479