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NCI-H716 [H716]

規格:

價格:

編號:B165360

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

NCI-H716 [H716]

商品貨號

B165360

Organism

Homo sapiens, human

Tissue

cecum

培養基

Product Format

frozen

Morphology

epithelial

Culture Properties

suspension, multicell aggregates and some adherent cells

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

colorectal adenocarcinoma

Age

33 years

Gender

male

Ethnicity

Caucasian

Storage Conditions

liquid nitrogen vapor phase

Karyotype

modal number = 61; range = 55 to 64.
This is a hypotriploid human cell line. The modal chromosome number is 61, occurring in 28% of cells. The rate of higher ploidies is 1.2%. Twelve marker chromosomes are common in these cells. These include t(7q,16q), t(5p,7p), t(4q,9q) and 9 others. A large number of DM's is found in every cell. The N21 is absent; and N9, N11, N13, N20, N22 and X are single-copied. No Y chromosome was detected in the Q-banded slide preparation.

Images

Derivation

This line was derived from cells present in ascites fluid obtained from a patient after treatment with 5-fluorouracil.

Clinical Data

33 years

Caucasian

male

Tumorigenic

Yes

Effects

Yes, in nude mice

(Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells)

Comments

The cells contain Dopa decarboxylase and, unlike other colorectal lines, contain cytoplasmic dense core granules characteristic of endocrine secretion.

The cells do not express the TAG-72 or CA19-9 antigens nor do they produce carcinoembryonic antigen (CEA).

Complete Growth Medium

The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.

Subculturing

Cultures can be maintained by the addition of fresh medium or replacement of medium Cultures can be established by centrifugation with subsequent resuspension at 4 to 8 x 10⁵ viable cells/mL.

Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended

Medium Renewal: Every 2 to 3 days

Cryopreservation

Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Atmosphere: air, 95%; carbon dioxide (CO₂), 5%

Temperature: 37°C

STR Profile

Amelogenin: X

CSF1PO: 11

D13S317: 8,11

D16S539: 11,12

D5S818: 11

D7S820: 10,11

THO1: 6,9.3

TPOX: 8,11

vWA: 16,17

Isoenzymes

AK-1, 1

ES-D, 1

G6PD, B

GLO-I, 1-2

Me-2, 1

PGM1, 1

PGM3, 1-2

Name of Depositor

AF Gazdar

Deposited As

Homo sapiens

Year of Origin

1984

References

Park JG, et al. Characteristics of cell lines established from human colorectal carcinoma. Cancer Res. 47: 6710-6718, 1987. PubMed: 3479249

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