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NCI-H747 [H747]

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價格:

編號:B165366

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

NCI-H747 [H747]

商品貨號

B165366

Organism

Homo sapiens, human

Tissue

cecum

培養基

Product Format

frozen

Morphology

epithelial

Culture Properties

adherent, floating aggregates of round cells with some attached cells

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

colorectal adenocarcinoma

Age

69 years

Gender

male

Ethnicity

Caucasian

Storage Conditions

liquid nitrogen vapor phase

Karyotype

modal number = 66; range = 63 to 75
This is a hypotriploid human cell line. The modal chromosome number is 66, occurring in 20% of cells. The rate of higher ploidies is 1.6%. Twelve marker chromosomes are common to all cells; these include single copy for i(1q), i(7p), i(10q); two copies for i(3q) and two to three copies for der(12)t(12;13)(q24,33;q12). The N12, N13, X and Y have single copy each. The presence of the Y was confirmed by Q-banding.

Derivation

This line was derived from a metastasis in a common duct node from a patient prior to therapy.

Clinical Data

69 years

Caucasian

male

Antigen Expression

Blood type A; Rh+; CA19-9 antigen

Genes Expressed

carcinoembryonic antigen (CEA), 8 ng/mL per 10⁶ cells per 10 days

Cellular Products

carcinoembryonic antigen (CEA), 8 ng/ml per 10 exp6 cells per 10 days

Tumorigenic

Yes

Effects

Yes, in nude mice

Tumors developed in 3 of 5 nude mice inoculated subcutaneously with 10⁷ cells

Comments

The cells are negative for Dopa decarboxylase and TAG-72 antigen but are positive for CEA and CA19-9 antigen.

Complete Growth Medium

The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.

Subculturing

Volumes are given for a 75 cm² flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.

Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Medium Renewal: Twice per week

Cryopreservation

Freeze medium: Complete growth medium supplemented with 5% DMSO

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Temperature: 37°C

Isoenzymes

AK-1, 1

ES-D, 1

G6PD, B

GLO-I, 2

Me-2, 1

PGM1, 1

PGM3, 1

Name of Depositor

AF Gazdar

Deposited As

Homo sapiens

References

Park JG, et al. Characteristics of cell lines established from human colorectal carcinoma. Cancer Res. 47: 6710-6718, 1987. PubMed: 3479249

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