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NF-1
NF-1
規(guī)格:
價格:
編號:B165390
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
NF-1
商品貨號
B165390
Organism
Mus musculus, mouse
Product Format
frozen
培養(yǎng)基
Morphology
epithelial
Culture Properties
mixed adherent-suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
nullipotent embryonal carcinoma; teratocarcinoma
Age
embryo; 7 days gestation
Gender
male
Strain
BALB/c
Applications
This pluripotent stem cell line was established in 1979 by Fox et al.
Derivation
This pluripotent stem cell line was established in 1979 by Fox et al.
Clinical Data
When injected into the tail veins of female mice, the cells have a tendency to metastasize to the ovaries and adrenals.
When injected into male mice, fewer, and slower growing, tumors are formed.
male
Tumorigenic
Yes
Effects
Yes, when injected intramuscularly into syngeneic mice
Comments
This pluripotent stem cell line was established in 1979 by Fox et al.
When injected into the tail veins of female mice, the cells have a tendency to metastasize to the ovaries and adrenals.
When injected into male mice, fewer, and slower growing, tumors are formed.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended
This is a mixed cell culture; cells grow both as a monolayer and as a suspension. Subcultures are prepared by scraping the attached cell into the medium containing the floaters. Aspirate and dispense into new culture flasks. Subculture approximately two times weekly. Cells will detach from surface of flask before confluency is reached.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Name of Depositor
I Damjanov
Deposited As
Mus musculus
Year of Origin
1979
References

Fox N, et al. Male murine embryonal carcinoma cell line selectively metastatic to the ovaries and adrenals. Virchow's Arch. B Cell Pathol. 43: 241-251, 1983. PubMed: 6137898

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