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NFS-5 C-1
NFS-5 C-1
規格:
價格:
編號:B165393
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
NFS-5 C-1
商品貨號
B165393
Organism
Mus musculus, mouse
Cell Type
pre-B lymphoblast
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
lymphoma
Strain
NFS/N
Applications
The NFS-5 C-1 cell line was derived from a lymphoma arising in a mouse inoculated with CAS- 2SM ecotropic murine leukemia virus.
They are negative for ThB, surface immunoglobulin (sIg) and Ia.
Tested and found negative for ectromelia virus (mousepox).
Derivation
The NFS-5 C-1 cell line was derived from a lymphoma arising in a mouse inoculated with CAS- 2SM ecotropic murine leukemia virus.
Antigen Expression
CD5 +; CD32 +; CD45R +
Genes Expressed
ecotropic retrovirus; mink cell focus forming retrovirus,CD5 +; CD32 +; CD45R +
Cellular Products
ecotropic retrovirus; mink cell focus forming retrovirus
Comments
The NFS-5 C-1 cell line was derived from a lymphoma arising in a mouse inoculated with CAS- 2SM ecotropic murine leukemia virus.
The cells bear a pattern of markers consistent with identification as large pre-B lymphoblasts (Ly 17, CD32.
Ly 1, CD5.
Lyb 2, Ly 5(B220), CD45R).
They are negative for ThB, surface immunoglobulin (sIg) and Ia.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
Modified Dulbecco's medium with 0.002 mg/ml transferrin and 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Maintain cell concentration between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Name of Depositor
WJ Davidson, HC Morse
Deposited As
Mus musculus
References

Davidson WF, et al. A unique series of lymphomas related to the Ly-1+ lineage of B lymphocyte differentiation. J. Immunol. 133: 744-753, 1984. PubMed: 6330201

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