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OV-90
OV-90
規格:
價格:
編號:B165444
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
OV-90
商品貨號
B165444
Organism
Homo sapiens, human
Tissue
ovary; derived from metastatic site: ascites
培養基
Product Format
frozen
Morphology
epithelial
Culture Properties
adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
grade 3, stage IIIC, malignant papillary serous adenocarcinoma
Age
64 years
Gender
female
Storage Conditions
liquid nitrogen vapor phase
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Karyotype
46, XX, der(1)t(1;10)(p36;p15), hsr(3)(p11), der(9;17)(q10;q10), der(10)t(10;17)(p15;p12p13), der(13)t(13;13)(p11;q14) RefProvencher DM, et al. Characterization of four novel epithelial cancer cell lines. In Vitro Cell. Dev. Biol. Anim. 36: 357-361, 2000. PubMed: 10949993
Images
Derivation
This cell line was initiated in August of 1992 from a patient of French-Canadian descent with no family history of ovarian cancer.
Clinical Data
female
Oncogene
her2/neu +, p53 (mutated, Ser --> Arg mutation at exon 6, codon 215) RefProvencher DM, et al. Characterization of four novel epithelial cancer cell lines. In Vitro Cell. Dev. Biol. Anim. 36: 357-361, 2000. PubMed: 10949993
Genes Expressed
keratin RefProvencher DM, et al. Characterization of four novel epithelial cancer cell lines. In Vitro Cell. Dev. Biol. Anim. 36: 357-361, 2000. PubMed: 10949993
Cellular Products
keratin
Tumorigenic
Yes
Effects
Yes, the cells are tumorigenic in nude mice and form colonies in soft agar
Comments
Like TOV-21G (ATCC CRL-11730), the OV-90 (ATCC CRL-11732) cell line exhibits a deletion at chromosome 3p24. The TOV-112D (ATCC CRL-11731) cell line does not exhibit a deletion at chromosome 3p24.
Complete Growth Medium
The base medium for this cell line is a 1:1 mixture of MCDB 105 medium containing a final concentration of 1.5 g/L sodium bicarbonate and Medium 199 containing a final concentration of 2.2 g/L sodium bicarbonate. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 15%

    • Subculturing
    Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
    1. Remove and discard culture medium.
    2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
    3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
      Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
    4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
    5. Add appropriate aliquots of the cell suspension to new culture vessels.
    6. Incubate cultures at 37°C.
      Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
      Medium Renewal: Every 3 to 4 days
      Cryopreservation
      Freeze Medium: Complete growth medium supplemented with 5% (v/v) DMSO
      Storage Temperature: Liquid nitrogen vapor phase
      Culture Conditions
      Temperature: 37°C
      Atmosphere: Air, 95%; carbon dioxide (CO2), 5%
      STR Profile
      Amelogenin: X
      CSF1PO: 12,13
      D13S317: 11,12
      D16S539: 11
      D5S818: 11,15
      D7S820: 10,10.1
      THO1: 9.3
      TPOX: 8,10
      vWA: 16,17
      Name of Depositor
      University of Montreal
      Deposited As
      human
      U.S. Patent Number
      Year of Origin
      August, 1992
      References

      Mes-Masson AM, Provencher D. Primary cultures of normal and tumoral human ovarian epithelium. US Patent 5,710,038 dated Jan 20 1998

      Provencher DM, et al. Characterization of four novel epithelial cancer cell lines. In Vitro Cell. Dev. Biol. Anim. 36: 357-361, 2000. PubMed: 10949993

      Lounis H, et al. Mapping of chromosome 3p deletions in human epithelial ovarian tumors. Oncogene 17: 2359-2365, 1998. PubMed: 9811467

      Provencher DM, et al. Characterization of four novel epithelial cancer cell lines. In Vitro Cell. Dev. Biol. Anim. 36: 357-361, 2000. PubMed: 10949993

      Provencher DM, et al. Characterization of four novel epithelial cancer cell lines. In Vitro Cell. Dev. Biol. Anim. 36: 357-361, 2000. PubMed: 10949993

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