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P388D1 [P388 D1]
P388D1 [P388 D1]
規(guī)格:
價(jià)格:
編號:B165453
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
P388D1 [P388 D1]
商品貨號
B165453
Organism
Mus musculus, mouse
Cell Type
monocyte; macrophage
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
lymphoma
Strain
DBA/2
Applications
transfection host
Storage Conditions
liquid nitrogen vapor phase
Tumorigenic
Yes
Effects

Yes, in nude mice

Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells. 

Comments

A subclone of this line [see ATCC TIB-63, P388D1(IL-1)] produces high levels of interleukin-1 (IL-1, interleukin 1). Tested and found negative for ectromelia virus (mousepox).

Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%.
Subculturing
Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 2 - 5 x 105 viable cells/mL.
A maximum of 6 x 106 viable cells/mL is obtainable.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Name of Depositor
EM Jensen
Deposited As
Mus musculus
References

Dawe CJ, Potter M. Morphologic and biologic progression of a lymphoid neoplasm of the mouse in vivo and in vitro. Am. J. Pathol. 33: 603, 1957

Standard Practice for Testing for Biological Responses to Particles in Vitro. West Conshohocken, PA:ASTM International;ASTM Standard Test Method F 1903-98R03.

Cross References

Nucleotide (GenBank) : U88624 Mus musculus 85 kDa calcium-independent phospholipase A2 mRNA, complete cds.

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