Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications
The antibody reacts with human plasma fibronectin and cross-reacts with cellular fibronectin.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Animals were immunized with purified human plasma fibronectin.
Spleen cells were fused with P3NP myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human fibronectin
Cellular Products
immunoglobulin; monoclonal antibody; against human fibronectin
Comments
Animals were immunized with purified human plasma fibronectin.
Spleen cells were fused with P3NP myeloma cells.
The antibody reacts with human plasma fibronectin and cross-reacts with cellular fibronectin.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
Culture Medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 90%; fetal bovine serum, 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
