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P3X63Ag8U.1

規格:

價格:

編號:B165459

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

P3X63Ag8U.1

商品貨號

B165459

Organism

Mus musculus, mouse

Cell Type

B lymphoblast

培養基

Product Format

frozen

Morphology

lymphoblast

Culture Properties

suspension

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

plasmacytoma; myeloma

Gender

female

Strain

BALB/c

Storage Conditions

liquid nitrogen vapor phase

Derivation

The line was derived from the MOPC 21 - P3X63Ag8 lineage.

Clinical Data

female

Antigen Expression

H-2d

Comments

The cells do not synthesize gamma 1 chains.

They produce but do not secrete kappa chains.

Tested and found negative for ectromelia virus (mousepox).

Complete Growth Medium

The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing

Cultures can be maintained by addition or replacement of fresh medium. Establish new cultures at 4 x 10⁵ viable cells/mL and maintain below 1 x 10⁶ cells/mL.

Medium Renewal: 3 times per week

Cryopreservation

Freeze medium: Culture medium, 95%; DMSO, 5%.

Culture Conditions

Temperature: 37°C

Name of Depositor

MD Scharff

Deposited As

Mus musculus

References

Yelton DE, et al. Fusion of mouse myeloma and spleen cells. Curr. Top. Microbiol. Immunol. 81: 1-7, 1978. PubMed: 567551

Cross References

Nucleotide (GenBank) : E02974 DNA encoding mouse interleukin-6(IL-6) receptor.

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