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PA317 LXSN
PA317 LXSN
規格:
價格:
編號:B165470
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
PA317 LXSN
商品貨號
B165470
Organism
Mus musculus, mouse
Tissue
embryo
培養基
Product Format
frozen
Morphology
fibroblast
Culture Properties
adherent
Biosafety Level
2 Cells contain human papilloma viral DNA sequences

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
Leukemia
Age
embryo
Strain
NIH/Swiss
Applications
Virions produced from the transfected Psi-2 cells were used to infect the amphotropic packaging line PA317, and infected cells were selected in medium containing G418.
PA317 LXSN cells produce an amphoteric retrovirus with an empty neo control vector, 5' long terminal repeat (LTR) from the Moloney murine leukemia virus (MoMuLV) multiple cloning region and 3' LTR from MoMuLV.
The vector also contains a gene controlling resistance to neomycin transcribed from the SV40 promoter.
This line is useful as a negative control for the use of PA317 LXSN 16E6E7 (see ATCC CRL-2203).
Derivation
PA317 LXSN is a packaging cell line developed by transfection of the retrovirus vector pLXSN into the Psi-2 ecotropic packaging cell line.
Virions produced from the transfected Psi-2 cells were used to infect the amphotropic packaging line PA317, and infected cells were selected in medium containing G418.
Comments
PA317 LXSN is a packaging cell line developed by transfection of the retrovirus vector pLXSN into the Psi-2 ecotropic packaging cell line.
Virions produced from the transfected Psi-2 cells were used to infect the amphotropic packaging line PA317, and infected cells were selected in medium containing G418.
PA317 LXSN cells produce an amphoteric retrovirus with an empty neo control vector, 5' long terminal repeat (LTR) from the Moloney murine leukemia virus (MoMuLV) multiple cloning region and 3' LTR from MoMuLV.
The vector also contains a gene controlling resistance to neomycin transcribed from the SV40 promoter.
This line is useful as a negative control for the use of PA317 LXSN 16E6E7 (see ATCC CRL-2203).
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:12 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, rinse flask with fresh 0.25% trypsin, 0.02% EDTA and remove trypsin, Add an additional 1 to 2 ml of trypsin solution, and allow the flask to sit at room temperature (or 37C) until the cells detach.
Add fresh medium, aspirate and dispense into new flasks.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Name of Depositor
DA Galloway
Deposited As
Mus musculus
References

Halbert CL, et al. The E7 gene of human papillomavirus type 16 is sufficient for immortalization of human epithelial cells. J. Virol. 65: 473-478, 1991. PubMed: 1845902

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