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PI 153/3
PI 153/3
規格:
價格:
編號:B165508
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
PI 153/3
商品貨號
B165508
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
Neuroblastoma,Leukemia
Applications
The antigen recognized by this antibody is present on fetal brain, B cell and Null cell leukemias but not on adult brain.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with P3X63Ag8 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against a glycoprotein expressed on human neuroectoderm derived tumors (neuroblastoma, retinoblastoma and glioblastoma)
Cellular Products
immunoglobulin; monoclonal antibody; against a glycoprotein expressed on human neuroectoderm derived tumors (neuroblastoma, retinoblastoma and glioblastoma)
Comments
Animals were immunized with the human neuroblastoma line IMR6.
Spleen cells were fused with P3X63Ag8 myeloma cells.
The antigen recognized by this antibody is present on fetal brain, B cell and Null cell leukemias but not on adult brain.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.
Isotype
IgM
Name of Depositor
R Kennett
Deposited As
mouse (B cell); mouse (myeloma)
References

Kennett RH, Gilbert F. Hybrid myelomas producing antibodies against a human neuroblastoma antigen present on fetal brain. Science 203: 1120-1121, 1979. PubMed: 424740

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