最新中文字幕-精品国产99-国产精品福利在线观看-粉嫩av一区二区三区-日韩精品久久久久-美丽的姑娘国语视频-亚洲欧洲色-国产不卡毛片-三级黄毛片-免费福利在线-日韩av三区-欧美成年人网站-欧洲美女与动交ccoo-成人涩涩软件-秋霞影院午夜老牛影院-婷婷久久伊人-呦呦视频在线观看-www.天天综合-日韩精品高清视频-国产欧美综合在线-欧美日韩一区二区三区在线视频-a猛片免费播放-91九色蝌蚪国产-国产欧美日韩专区-嫩模被强到高潮呻吟不断

歷史搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當(dāng)前位置: 首頁 > ATCC代理 > PLC/PRF/5 B165513
PLC/PRF/5
PLC/PRF/5
規(guī)格:
價格:
編號:B165513
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
PLC/PRF/5
商品貨號
B165513
Organism
Homo sapiens, human
Tissue
liver
培養(yǎng)基
Cell Type
Alexander cells
Product Format
frozen
Morphology
epithelial
Culture Properties
adherent
Biosafety Level
2 [Cells contain hepatitis B]

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
hepatoma
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Images
ATCC CRL-8024 Cell Micrograph
Genes Expressed
hepatitis virus B surface antigen (HBsAg)
Cellular Products
hepatitis virus B surface antigen (HBsAg)
Comments
The line was originally contaminated with mycoplasma, and was cured by treatment with BM-cycline.
The cells secrete HBsAg.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Twice per week
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 10
D13S317: 11,12
D16S539: 13
D5S818: 12
D7S820: 9,11
THO1: 8
TPOX: 8
vWA: 15,16
Name of Depositor
WJ McAleer
Deposited As
Homo sapiens
U.S. Patent Number
References

Monjardino J, Crawford E. Polypeptide profile of HBSAg excreted by a human hepatoma cell line. Virology 96: 652-655, 1979. PubMed: 223321

MacNab GM, et al. Hepatitis B surface antigen produced by a human hepatoma cell line. Br. J. Cancer 34: 509-515, 1976. PubMed: 187208

Skelly J, et al. Hepatitis B surface antigen produced by a human hepatoma cell line. Nature 282: 617-618, 1979. PubMed: 233138

Knowles B. et al. Human hepatocellular carcinoma cell lines secrete the major plasma proteins and hepatitis B surface antigen. Science 209(4455): 497-499, 1980. PubMed: 6248960

Alexander J, et al. Adaptation of cells derived from human malignant tumours to growth in vitro. S. Afr. J. Med. Sci. 41(2):89-98, 1976. PubMed:184551

Gilligan A, et al. Variation in EGF-induced EGF receptor downregulation in human hepatoma- derived cell lines expressing different amounts of EGF receptor. Exp. Cell Res. 200: 235-241, 1992. PubMed: 1315281

Alexander JJ, et al. Establishment of a continuously growing cell line from primary carcinoma of the liver. S. Afr. Med. J. 50: 2124-2128, 1976. PubMed: 63998

Cross References

Nucleotide (GenBank) : X75311 H.sapiens /Hepatitis B virus fusion mRNA for mevalonate kinase.

Nucleotide (GenBank) : X77700 H.sapiens / Hepatitis B virus (A4.0') DNA for mevalonate kinase (partial).

  • 王經(jīng)理
    手機號:17280875617
    QQ:1438578920
  • 胡經(jīng)理
    手機號:13345964880
    QQ:2438244627
  • 周經(jīng)理
    手機號:17757487661
    QQ:1296385441
  • 于經(jīng)理
    手機號:18067160830
    QQ:2088210172
  • 沈經(jīng)理
    手機號:19548299266
    QQ:2662369050
  • 李經(jīng)理
    手機號:13626845108
    QQ:972239479