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PMJ2-PC

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價格:

編號:B165516

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

PMJ2-PC

商品貨號

B165516

Organism

Mus musculus, mouse

Cell Type

peritoneal macrophage; infected with J2 viru

培養基

Product Format

frozen

Morphology

macrophage

Culture Properties

suspension; some adherent cells

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Gender

female

Strain

C57BL/6J

Applications

Cell staining demonstrated intracellular expression of the product of the v-raf gene in these cell lines.

Analysis demonstrated the presence of peritoneal macrophage associated cell surface antigens, interleukin-6 (IL-6) secretion induced by lipopolysaccharide (LPS), and biological response modifier-induced cytotoxic activity against tumor cells.

The cells are phagocytic, non-specific esterase positive and are Fc receptor positive.

The cells produce nitric oxide (NO) when stimulated with a mixture of rMuIFN-gamma and LPS.

Storage Conditions

liquid nitrogen vapor phase

Clinical Data

female

Antigen Expression

MHC class II +; MAC-1 (CD11b) +; MAC-2 +; Fc receptor (FcR) +; F4/80 +; Ly-5 +; Lyt-1.1 - ; Lyt-1.2 -; Thy-1.2 -; sIg -

Genes Expressed

MHC class II +; MAC-1 (CD11b) +; MAC-2 +; Fc receptor (FcR) +; F4/80 +; Ly-5 +; Lyt-1.1 - ; Lyt-1.2 -; Thy-1.2 -; sIg -

Comments

PMJ2-PC (ATCC CRL-2457) and AMJ2-R (ATCC CRL-2458) are cloned, continuous, peritoneal macrophage cell lines generated from C57BL6J mice by in vivo infection with the J2 retrovirus carrying the v-raf and v-myc oncogenes.

Cell staining demonstrated intracellular expression of the product of the v-raf gene in these cell lines.

The in vivo immortalized cells had many of the morphological and functional characteristics of peritoneal macrophages.

The cells are phagocytic, non-specific esterase positive and are Fc receptor positive.

One of the clones, PMJ2-PC, constitutively expressed major histocompatibility complex (MHC) class II antigens, and in the other clone, PMJ2-R, MHC class II antigens expression was induced by recombinant murine interferon-gamma.

The cells produce nitric oxide (NO) when stimulated with a mixture of rMuIFN-gamma and LPS.

Complete Growth Medium

Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 5 mM HEPES, 95%; fetal bovine serum, 5%

Subculturing

Scrape off the attached cells and transfer along with the floating cells into new flasks.

Medium Renewal: Twice per week

Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions

Temperature: 37°C

Atmosphere: Air, 95%; Carbon dioxide (CO₂), 5%

Name of Depositor

AV Palleroni

Deposited As

mouse

References

Adami C, et al. In vivo immortalization of murine peritoneal macrophages: a new rapid and efficient method for obtaining macrophage cell lines. J. Leukocyte Biol. 53: 475-478, 1993. PubMed: 7683328

Palleroni AV, et al. Nitric oxide synthase induction in lines of macrophages from different anatomical sites. Cell. Mol. Biol. 44: 527-535, 1998. PubMed: 9620450

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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