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PS 38
PS 38
規(guī)格:
價格:
編號:B165523
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產品名稱
PS 38
商品貨號
B165523
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody cross-reacts with the NP-OT of both mouse in both radioimmunoassays and immunohistochemical assays.
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against rat oxytocin associated neurophysin (NP-OT)
Cellular Products
immunoglobulin; monoclonal antibody; against rat oxytocin associated neurophysin (NP-OT)
Comments
Animals were immunized with an acid soluble extract of rat (Sprague-Dawley) intermediate lobes.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody cross-reacts with the NP-OT of both mouse in both radioimmunoassays and immunohistochemical assays.
Complete Growth Medium
Dulbecco's modified Eagle's medium with 4.5 g/L glucose, non-essential amino acids, 2 mM L-glutamine and 1 mM sodium pyruvate, 90%; heat-inactivated fetal bovine serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype
IgG2b; kappa light chain
Name of Depositor
H Gainer
Deposited As
mouse (B cell); mouse (myeloma)
References

Ben-Barak Y, et al. Neurophysin in the hypothalamo-neurohypophysial system. I. Production and characterization of monoclonal antibodies. J. Neurosci. 5: 81-97, 1985. PubMed: 3880813

Ben-Barak Y, et al. Phylogenetic cross-reactivities of monoclonal antibodies produced against rat neurophysin. Cell. Mol. Neurobiol. 4: 339-349, 1984. PubMed: 6085284

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