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PtK2 (NBL-5)
PtK2 (NBL-5)
規(guī)格:
價(jià)格:
編號(hào):B165535
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
PtK2 (NBL-5)
商品貨號(hào)
B165535
Organism
Potorous tridactylus, potoroo
Tissue
kidney
培養(yǎng)基
Product Format
frozen
Morphology
epithelial
Culture Properties
adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
Normal
Age
adult
Gender
male
Applications
This cell line is a suitable transfection host.
Storage Conditions
liquid nitrogen vapor phase
Clinical Data
male
Genes Expressed
keratin
Cellular Products
keratin
Virus Susceptibility
Human Coxsackievirus A 9
Herpes simplex virus
Vaccinia virus
Vesicular stomatitis New Jersey virus
Virus Resistance
adenovirus 5; coxsackievirus B5; poliovirus 2
Comments

The cells are positive for keratin by immunoperoxidase staining.

Complete Growth Medium
The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing

Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin 0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. 
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach.  Cells that are difficult to detach may be placed at 37oC to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.  
  5. Add appropriate aliquots of the cell suspension into new culture vessels.
  6.  Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Name of Depositor
KH Whalen
Deposited As
Potorous tridactylis
References

Walen KH. Spatial Relationships in the Replication of Chromosomal DNA. Genetics 51: 915-929, 1965. PubMed: 14337764

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