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P-tyr-1
P-tyr-1
規格:
價格:
編號:B165537
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
P-tyr-1
商品貨號
B165537
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with phosphotyrosine and proteins containing phosphotyrosine.
The antibody does not react with phosphothreonine or phosphoserine, and is useful in immunoprecipitation and western blot assays.
Derivation
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against phosphotyrosine
Cellular Products
immunoglobulin; monoclonal antibody; against phosphotyrosine
Comments
Animals were immunized with aminobenzylphosphonate conjugated to hemocyanin.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts with phosphotyrosine and proteins containing phosphotyrosine.
The epitope recognized by P-tyr-1 appears to be on the phenyl phosphate group of phosphotyrosine.
The antibody does not react with phosphothreonine or phosphoserine, and is useful in immunoprecipitation and western blot assays.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype
IgG1
Name of Depositor
JK Klarlund
Deposited As
mouse (B cell); mouse (myeloma)
References

Klarlund JK. Transformation of cells by an inhibitor of phosphatases acting on phosphotyrosine in proteins. Cell 41: 707-717, 1985. PubMed: 2408760

Klarlund JK, et al. Numerous proteins phosphorylated on tyrosine and enhanced tyrosine kinase activities in vanadate-treated NIH 3T3 fibroblasts. Biochim. Biophys. Acta 971: 112-120, 1988. PubMed: 3408742

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