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PV1
PV1
規格:
價格:
編號:B165539
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
PV1
商品貨號
B165539
Organism
hamster (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Strain

Strain:? Armenian (B cell); BALB/c (myeloma)

Applications
The antibody reacts with mouse CD28 receptors.
The antibody is specific for a 90 kDa protein homodimer.
It reacts with all mature T cells and can both activate and inhibit T cell function.
It is a supplemental deposit of ATCC HB-11944. HB-11944 is viable but does not produce antibody.
Note: This cell line is viable and does secrete antibody at low levels.
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Spleen cells were fused with Sp2/0 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against mouse receptor for CD28
Cellular Products
immunoglobulin; monoclonal antibody; against mouse receptor for CD28
Comments
Animals were immunized with EL4 cells.
Spleen cells were fused with Sp2/0 myeloma cells.
The antibody reacts with mouse CD28 receptors.
The antibody is specific for a 90 kDa protein homodimer.
It reacts with all mature T cells and can both activate and inhibit T cell function.
Note: This cell line is viable and does secrete antibody at low levels. It is a supplemental deposit of ATCC HB-11944. HB-11944 is viable but does not produce antibody.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Add medium as pH drops
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10 exp5 viable cells/ml.
Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Do not allow cell concentration to exceed 1 X 10 exp6 cells/ml.
Cryopreservation
Complete growth medium supplemented with 5% (v/v) DMSO.
Culture Conditions
Temperature: 37.0°C
Isotype
IgG
Name of Depositor
Naval Medical Research Institute
Deposited As
hamster (B cell); mouse (myeloma)
U.S. Patent Number
References

Abe R, et al. Distinct signal transduction in mouse CD4+ and CD8+ splenic T cells after CD28 receptor ligation. J. Immunol. 154: 985-997, 1995. PubMed: 7822814

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