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R187
R187
規格:
價格:
編號:B165555
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
R187
商品貨號
B165555
Organism
Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Tissue
Spleen, kidney
培養基
Cell Type
Hybridoma: B lymphocyte
Product Format
frozen
Morphology
Lymphoblast
Culture Properties
Suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Strain
Osborne-Mendel (B cell); BALB/c (myeloma)
Applications
The antibody immunoprecipitates p30, binds efficiently to S. aureus protein A, and works well in Western blotting assays for p30. The antibody reacts with the p30 group antigen (gag) from most MuLV.
Storage Conditions
Liquid nitrogen vapor phase
Derivation
Animals were immunized with spleen focus forming virus (SFFV) infected nonproducer normal rat kidney (NRK) cells in complete Freund's adjuvant. Spleen cells were fused with NS-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against p30 gag protein of murine leukemia virus (MuLV)
Cellular Products
immunoglobulin; monoclonal antibody; against p30 gag protein of murine leukemia virus (MuLV)
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 10%
  • 0.05 mM 2-mercaptoethanol

Subculturing
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 105 cells/mL and maintain between 1 X 105 and 1 X 106 cells/mL.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
Isotype
rat IgG1
Name of Depositor
B Chesebro
Deposited As
rat (B cell); mouse (myeloma)
References

Chesebro B, et al. Characterization of monoclonal antibodies reactive with murine leukemia viruses: Use in analysis of strains of Friend MCF and Friend ecotropic murine leukemia virus. Virology 127: 134-148, 1983. PubMed: 6305011

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