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R2-10F6 [R2-10F6.3C10.3F9]

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編號:B165558

品牌:Mingzhoubio

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RNA

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凍干粉

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產品名稱

R2-10F6 [R2-10F6.3C10.3F9]

商品貨號

B165558

Organism

Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)

Cell Type

hybridoma: B lymphocyte

培養基

Product Format

frozen

Culture Properties

suspension

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications

The hybridoma cell line R2-10F6 [R2-10F6.3C10.3F9] was established by David Presky and Victoria Wilkinson in 1993 and subcloned twice.

This antibody is a useful tool for detection of mouse IL-12p40 by ELISA and Western Blot analysis.

Derivation

The hybridoma cell line R2-10F6 [R2-10F6.3C10.3F9] was established by David Presky and Victoria Wilkinson in 1993 and subcloned twice.

Clinical Data

R2-10F6 was formed by the fusion of SP2/0 mouse myeloma cells with splenocytes from an adult female Lewis rat inoculated with recombinant mouse IL-12 p75.

Genes Expressed

immunoglobulin; monoclonal antibody; against mouse interleukin 12 (IL-12) p40

Cellular Products

immunoglobulin; monoclonal antibody; against mouse interleukin 12 (IL-12) p40

Comments

The hybridoma cell line R2-10F6 [R2-10F6.3C10.3F9] was established by David Presky and Victoria Wilkinson in 1993 and subcloned twice.

This antibody is a useful tool for detection of mouse IL-12p40 by ELISA and Western Blot analysis.

R2-10F6 was formed by the fusion of SP2/0 mouse myeloma cells with splenocytes from an adult female Lewis rat inoculated with recombinant mouse IL-12 p75.

Complete Growth Medium

Iscove's Modified Dulbecco's Medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate supplemented with 0.05 mM 2-mercaptoethanol and 100 U/ml recombinant human IL-6, 90%; fetal bovine serum, 10%.

Subculturing

Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Alternately, cultures can be established by centrifugation with subsequent resuspension in fresh culture medium.

Establish new cultures at 2 X 10 exp5 viable cells/ml and maintain at between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.

Cryopreservation

Culture medium, 95%; DMSO, 5%

Culture Conditions

Temperature: 37.0°C

Isotype

IgG2b

Name of Depositor

DH Presky

Year of Origin

1993

References

Wilkinson VL, et al. Characterization of anti-mouse IL-12 monoclonal antibodies and measurement of mouse IL-12 by ELISA. J. Immunol. Methods 189: 15-24, 1996. PubMed: 8576576

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