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Ramos.2G6.4C10

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編號:B165573

品牌:Mingzhoubio

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產品名稱

Ramos.2G6.4C10

商品貨號

B165573

Organism

Homo sapiens, human

Cell Type

B lymphocyte

培養基

Product Format

frozen

Morphology

lymphoblast

Culture Properties

suspension

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

Burkitt's lymphoma (American)

Age

3 years

Gender

male

Ethnicity

Caucasian

Applications

Ramos.2G6.4C10 cells provide a sensitive and specific assay system for IL-4 that is insensitive to the presence of other lymphokines and cytokines such as interleukins (IL-2, IL-2 and IL-6), colony stimulating factors (G-CSF, M-CSF and GM-CSF) and interferons alpha and gamma (IFN alpha, IFN gamma). Tumor necrosis factors alpha and beta (TNF alpha, TNF beta) cause only minor interference.

The assay is sensitive to IL-4 concentrations in the range 50 to 800 pg/mL.

Storage Conditions

liquid nitrogen vapor phase

Derivation

This line was derived from the Ramos (RA-1) cell line (see ATCC CRL-1596) by repetitive cloning with selection for low constitutive expression of CD23 and maximum stimulation of CD23 expression by IL-4.

Clinical Data

3 years

Caucasian

male

Antigen Expression

CD23 +

Receptor Expression

interleukin 4 (IL-4); low affinity IgE (CD23)

Genes Expressed

immunoglobulin (surface and secreted)

Cellular Products

immunoglobulin (surface and secreted)

Comments

Both the parental line (ATCC CRL-1596) and the derivative cell line Ramos.2G6.4C10 (ATCC CRL-1923) have about 1500 IL-4 binding sites per cell.

Complete Growth Medium

The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.

Subculturing

Cultures can be maintained by addition or replacement of fresh medium. Establish new cultures at 5 x 10 ⁵ viable cells/mL and maintain between 1 and 2 x 10 ⁶ cells/mL.

Medium Renewal: Add fresh medium every 4 to 6 days (depending on cell density)

Cryopreservation

Freeze medium: Complete growth medium, 92.5%; DMSO, 7.5%

Storage temperature: liquid nitrogen vapor temperature

Culture Conditions

Temperature: 37°C

STR Profile

Amelogenin: X

CSF1PO: 10,11

D13S317: 13

D16S539: 10,13

D5S818: 7,12

D7S820: 11

THO1: 7,9.3

TPOX: 8,9

vWA: 15,16

Isotype

IgM

Name of Depositor

JP Siegel

Deposited As

Homo sapiens

References

Klein G, et al. An EBV-genome-negative cell line established from an American Burkitt lymphoma; receptor characteristics. EBV infectibility and permanent conversion into EBV-positive sublines by in vitro infection. Intervirology 5: 319-334, 1975. PubMed: 181343

Siegel JP, Mostowski HS. A bioassay for the measurement of human interleukin-4. J. Immunol. Methods 132: 287-295, 1990. PubMed: 1698879

Rousset F, et al. Regulation of Fc receptor for IgE (CD23) and class II MHC antigen expression on Burkitt's lymphoma cell lines by human IL-4 and IFN-gamma. J. Immunol. 140: 2625-2632, 1988. PubMed: 2965726

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