最新中文字幕-精品国产99-国产精品福利在线观看-粉嫩av一区二区三区-日韩精品久久久久-美丽的姑娘国语视频-亚洲欧洲色-国产不卡毛片-三级黄毛片-免费福利在线-日韩av三区-欧美成年人网站-欧洲美女与动交ccoo-成人涩涩软件-秋霞影院午夜老牛影院-婷婷久久伊人-呦呦视频在线观看-www.天天综合-日韩精品高清视频-国产欧美综合在线-欧美日韩一区二区三区在线视频-a猛片免费播放-91九色蝌蚪国产-国产欧美日韩专区-嫩模被强到高潮呻吟不断

歷史搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > RD B165586
RD
RD
規格:
價格:
編號:B165586
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
RD
商品貨號
B165586
Organism
Homo sapiens, human
Tissue
muscle
培養基
Product Format
frozen
Morphology
spindle cells and large multinucleated cells
Culture Properties
adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
rhabdomyosarcoma
Age
7 years
Gender
female
Ethnicity
Caucasian
Applications
This cell line can be used for the detection of viruses.
Storage Conditions
liquid nitrogen vapor phase
Karyotype
unstable within a hyperdiploid bimodal stemline number of 49 and 50. Twenty-two cells had chromosome associations, 15 cells microchromosomes, 2 cells had fragments, 2 cells with breaks, 2 cells with achromatic gaps and 1 cell with a secondary constriction Note: Cytogenetic information is based on initial seed stock at ATCC. Cytogenetic instability has been reported in the literature for some cell lines.
Images
Clinical Data
7 years
Caucasian
female
Genes Expressed
myoglobin; myosin ATPase
Cellular Products
myoglobin
myosin ATPase
Virus Susceptibility
Human poliovirus 1
Vesicular stomatitis, Glasgow (Indiana)
Vesicular stomatitis, Orsay (Indiana)
Herpes simplex virus
Vaccinia virus
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing

Volumes used in this protocol are for a 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum, which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 10 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. 
  5. Add appropriate aliquots of the cell suspension to new culture vessels. 
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Every 3 to 4 days
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 10,11
D13S317: 13
D16S539: 10,11
D5S818: 11
D7S820: 8,12
THO1: 9.3
TPOX: 9
vWA: 18
Isoenzymes
G6PD, B
Name of Depositor
RM McAllister
Deposited As
Homo sapiens
References

American Public Health Association. Compendium of methods for the microbiological examination of foods. 3rd ed.Washington, DC: American Public Health Association; 1992.

McAllister RM, et al. Cultivation in vitro of cells derived from a human rhabdomyosarcoma. Cancer 24: 520-526, 1969. PubMed: 4241949

Chen TR, et al. Cell identity resolved. Nature 340: 106, 1989. PubMed: 2739733

Stratton MR, et al. Misidentified cell. Nature 337: 311-312, 1989. PubMed: 2911385

Gershwin ME, et al. Immunobiology of heterotransplanted human tumors in nude mice. J. Natl. Cancer Inst. 58: 1455-1463, 1977. PubMed: 857033

Hahon N, Cooke KO. Primary virus-cell interactions in the immunofluorescence assay of Venezuelan equine encephalomyelitis virus. J. Virol. 1: 326, 1967. PubMed: 4918236

Guerin E, et al. Stromelysin-3 induction and interstitial collagenase repression by retinoic acid. J. Biol. Chem. 272: 11088-11095, 1997. PubMed: 9111003

Jeffers M, et al. Degradation of the Met tyrosine kinase receptor by the ubiquitin-proteasome pathway. Mol. Cell. Biol. 17: 799-808, 1997. PubMed: 9001234

Massuda ES, et al. Regulated expression of the diphtheria toxin A chain by a tumor-specific chimeric transcription factor results in selective toxicity for alveolar rhabdomyosarcoma cells. Proc. Natl. Acad. Sci. USA 94: 14701-14706, 1997. PubMed: 9405676

Tiger CF, et al. Presence of laminin alpha5 chain and lack laminin alpha1 chain during human muscle development and in muscular dystrophies. J. Biol. Chem. 272: 28590-28595, 1997. PubMed: 9353324

Sabbioni S, et al. GOK: a gene at 11p15 involved in rhabdomysarcoma and rhabdoid tumor development. Cancer Res. 57: 4493-4497, 1997. PubMed: 9377559

Xiao GH, et al. The Tuberous Sclerosis 2Gene Product, Tuberin, Functions as a Rab5 GTPase Activating Protein (GAP) in Modulating Endocytosis. J. Biol. Chem. 272: 6097-6100, 1997. PubMed: 9045618

Cross References

Nucleotide (GenBank) : U09279 Human type XIX collagen (COL19A1) mRNA, partial cds.

  • 王經理
    手機號:17280875617
    QQ:1438578920
  • 胡經理
    手機號:13345964880
    QQ:2438244627
  • 周經理
    手機號:17757487661
    QQ:1296385441
  • 于經理
    手機號:18067160830
    QQ:2088210172
  • 沈經理
    手機號:19548299266
    QQ:2662369050
  • 李經理
    手機號:13626845108
    QQ:972239479