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RF/6A
RF/6A
規(guī)格:
價格:
編號:B165589
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
RF/6A
商品貨號
B165589
Organism
Macaca mulatta, monkey, rhesus
Tissue
choroid; retina
培養(yǎng)基
Cell Type
endothelial
Product Format
frozen
Morphology
epithelial
Culture Properties
adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age
fetus
Applications
Late passage cells have been used as feeder layers to enhance attachment, subcultivation and maintenance of uveal melanocytes.
Storage Conditions
liquid nitrogen vapor phase
Derivation
This line spontaneously transformed at an early age and has been passaged over 540 times.
Genes Expressed
factor VIII; fibronectin
Comments
Weibel-Palade granules are seen by electron microscopy.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Remove medium, add fresh 0.25% trypsin, 0.53 mM EDTA solution for 2 minutes at room temperature, remove trypsin and let the culture sit at 37°C for 5 minutes (or until the cells detach). Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Twice weekly
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Culture Conditions
Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Name of Depositor
F Hu
Deposited As
Macaca mulatta
References

Lou DA, Hu FN. Specific antigen and organelle expression of a long-term rhesus endothelial cell line. In Vitro Cell. Dev. Biol. 23: 75-85, 1987. PubMed: 3102454

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