Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
The cells can be transformed with the RAS and GLI oncogenes.
The cells have an essentially normal karyotype with the exception of one 5q.
Transformation with RAS or GLI renders the cells tumorigenic.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
Kinzler KW, et al. Identification of an amplified, highly expressed gene in a human glioma. Science 236: 70-73, 1987. PubMed: 3563490
Kinzler KW, Vogelstein B. The GLI gene encodes a nuclear protein which binds specific sequences in the human genome. Mol. Cell. Biol. 10: 634-642, 1990. PubMed: 2105456
Kinzler KW, et al. The GLI gene is a member of the Kruppel family of zinc finger proteins. Nature 332: 371-374, 1988. PubMed: 2832761
