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RmcB
RmcB
規格:
價格:
編號:B165615
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
RmcB
商品貨號
B165615
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with human CAR (the receptor for coxsackie B virus and the binding protein for adenovirus 2 and 3).
Storage Conditions
liquid nitrogen vapor phase
Derivation
A/J mice were immunized with a 50-kilodalton receptor protein (Rp-a) for the group B coxsackieviruses (CB) isolated in a virus-receptor complex from detergent-solubilized HeLa cells.
Spleen cells were fused with Sp2/0 myeloma cells.
The RmcB cell line was established in 1985 by R. L. Crowell.
Genes Expressed
immunoglobulin; monoclonal antibody; against human CAR (Coxsackie - Adenovirus Receptor)
Cellular Products
immunoglobulin; monoclonal antibody; against human CAR (Coxsackie - Adenovirus Receptor)
Comments
A/J mice were immunized with a 50-kilodalton receptor protein (Rp-a) for the group B coxsackieviruses (CB) isolated in a virus-receptor complex from detergent-solubilized HeLa cells.
Spleen cells were fused with Sp2/0 myeloma cells.
The RmcB cell line was established in 1985 by R. L. Crowell.
The antibody reacts with human CAR (the receptor for coxsackie B virus and the binding protein for adenovirus 2 and 3).
The antibody blocks binding and infection by coxsackie B Virus, but not adenovirus.
Mouse monoclonal antibody (RmcB) protects HeLa cells and Buffalo green monkey kidney cells from infection by all six serotypes of CB. RmcB did not protect HeLa cells from infection by poliovirus, echovirus 6, or coxsackievirus A18.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing
Cultures can be maintained by addition of fresh Medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 x 105 viable cells/mL.  Maintain cultures at a cell concentration between 1 x 105 and 9 x 105 cells/mL. Do not allow the cell concentration to exceed 9 x 105  cells/mL.
Medium Renewal:  Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Isotype
IgG1
Name of Depositor
RW Finberg
Deposited As
mouse (B cell); mouse (myeloma)
Year of Origin
1985
References

Bergelson JM, et al. Isolation of a common receptor for coxsackie B viruses and adenoviruses 2 and 5. Science 275: 1320-1323, 1997. PubMed: 9036860

Mapoles JE, et al. Purification of a HeLa cell receptor protein for group B coxsackieviruses. J. Virol. 55: 560-566, 1985. PubMed: 2991580

Hsu KH, et al. A monoclonal antibody specific for the cellular receptor for the group B coxsackieviruses. J. Virol. 62: 1647-1652, 1988. PubMed: 2451756

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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