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RS4;11
RS4;11
規格:
價格:
編號:B165633
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
RS4;11
商品貨號
B165633
Organism
Homo sapiens, human
Tissue
bone marrow
培養基
Cell Type
lymphoblast
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
acute lymphoblastic leukemia
Age
32 years
Gender
female
Ethnicity
Caucasian
Storage Conditions
liquid nitrogen vapor phase
Karyotype
The cells have a characteristic chromosomal abnormality, t(4;11)(q21;q23), and an isochromosome for the long arm of chromosome 7.
Derivation
This line was established from the bone marrow of a patient with acute lymphoblastic leukemia.
Clinical Data
32 years
Caucasian
female
Antigen Expression
HLA DR +; CD9 +; CD24 +
Comments
The cells lack surface and cytoplasmic immunoglobulin, and are negative for CALLA (CD10).

The cells are also negative for myeloperoxidase and chloroacetate esterase and do not stain with Sudan Black. The cells are strongly positive for terminal deoxynucleotidyl transferase (TdT) and are positive for BA-1 (CD24), BA-2 (CD9) and MHC Class II antigens (HLA DR).

 

Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 105 viable cells/mL. Maintain cell density between 1 X 105 and 1 X 106 viable cells/mL.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze Medium: Complete growth medium, 95%; DMSO, 5%
Storage Temperature: Liquid nitrogen vapor temperature
Culture Conditions
Atmosphere: Air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 11,12
D13S317: 11
D16S539: 11,12
D5S818: 12,13
D7S820: 8,12
THO1: 6,9.3
TPOX: 8
vWA: 14,17
Name of Depositor
JH Kersey
Deposited As
Homo sapiens
References

Stong RC, et al. Human acute leukemia cell line with the t(4;11) chromosomal rearrangement exhibits B lineage and monocytic characteristics. Blood 65: 21-31, 1985. PubMed: 3917311

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