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RTH-149

規格:

價格:

編號:B165639

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

RTH-149

商品貨號

B165639

Organism

Oncorhynchus mykiss, trout, rainbow

Tissue

liver

培養基

Product Format

frozen

Morphology

epithelial

Culture Properties

adherent

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

hepatoma

Storage Conditions

liquid nitrogen vapor temperature

Comments

Culture the cells at 21C.

Complete Growth Medium

The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing

Protocol: Remove medium, rinse with fresh 0.25% trypsin, 0.02% EDTA solution, remove trypsin and let the culture sit at room temperature until the cells detach (about 5 minutes). Add fresh medium, aspirate and dispense into new flasks.

Subcultivation Ratio: A subcultivation ratio of 1:3 is recommended

Medium Renewal: Twice per week

Cryopreservation

Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor temperature

Culture Conditions

Temperature: 21.0°C

Name of Depositor

JL Fryer

Deposited As

Salmo gairdneri

References

Lannan CN, et al. Fish cell lines: establishment and characterization of nine cell lines from salmonids. In Vitro 20: 671-676, 1984. PubMed: 6542066

Fryer J, et al. A cell line derived from rainbow trout (Salmo gairdneri) hepatoma. Fish Pathol 15:193-200, 1981.

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