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S4B6-1

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編號:B165655

品牌:Mingzhoubio

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產品名稱

S4B6-1

商品貨號

B165655

Organism

Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)

Cell Type

hybridoma: B lymphocyte

培養基

Product Format

frozen

Morphology

lymphoblast

Culture Properties

suspension

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Strain

Strain:? Lewis (B cell); BALB/c (myeloma)

Applications

This is a supplemental deposit of the S4B6 hybridoma (ATCC HB-8794) that has improved antibody production.

The cells secrete immunoglobulin (rat IgG2a) for a short period in culture.

The antibody binds to mouse IL-2 and neutralizes mouse IL-2 activity.

Storage Conditions

liquid nitrogen vapor phase

Disclosure

This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

Derivation

Spleen cells were fused with Sp2/0 myeloma cells.

Genes Expressed

immunoglobulin; monoclonal antibody; against mouse interleukin 2 (interleukin-2, IL-2)

Cellular Products

immunoglobulin; monoclonal antibody; against mouse interleukin 2 (interleukin-2, IL-2)

Tumorigenic

Yes

Effects

Yes, forms ascites in pristane primed and immunosuppressed BALB/c mice (irradiated 150R to 450 R)

Comments

This is a supplemental deposit of the S4B6 hybridoma (ATCC HB-8794) that has improved antibody production.

Animals were immunized with supernatant from a mouse helper T cell line (LB2-1, see ATCC CRL-8629).

Spleen cells were fused with Sp2/0 myeloma cells.

The cells secrete immunoglobulin (rat IgG2a) for a short period in culture.

To grow as ascites, inject 2 X 10 exp6 cells/ml into BALB/c mice primed one week earlier with 0.5 ml pristane and given 150R to 450R of gamma irradiation.

The antibody binds to mouse IL-2 and neutralizes mouse IL-2 activity.

It does not react with rat or human IL-2.

Complete Growth Medium

The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.

Subculturing

Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10(5) cells/ml and maintain between 1 X 10(5) and 2 X 10(6) cells/ml.

Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)

Cryopreservation

Freeze medium: culture medium 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Isotype

IgG2a

Name of Depositor

DNAX Research Institute of Molecular and Cellular Biology, Inc.

Deposited As

rat (B cell); mouse (myeloma)

U.S. Patent Number

4,690,893

References

Mosmann TR. Hybridoma cell lines producing monoclonal antibodies which specifically bind to mouse interleukin-2. US Patent 4,690,893 dated Sep 1 1987

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