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S6F1
S6F1
規(guī)格:
價(jià)格:
編號(hào):B165657
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng)
S6F1
商品貨號(hào)
B165657
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
Animals were immunized with the 1670 cell line produced by immortalizing spleen cells from a whitelip tamarin with Herpesvirus saimiri.
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Animals were immunized with the 1670 cell line produced by immortalizing spleen cells from a whitelip tamarin with Herpesvirus saimiri.
Spleen cells were fused with P3/NSI/1-Ag4-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against a subset of human CD8 (LFA-1) positive T cells
Cellular Products
immunoglobulin; monoclonal antibody; against a subset of human CD8 (LFA-1) positive T cells
Comments
Animals were immunized with the 1670 cell line produced by immortalizing spleen cells from a whitelip tamarin with Herpesvirus saimiri.
Spleen cells were fused with P3/NSI/1-Ag4-1 myeloma cells.
The antibody recognizes a subset of CD8 positive T cells that have cytotoxic effector activity.
The S6F1 epitope is absent on CD8 positive suppressor cells.
Complete Growth Medium
Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 2 mM L-glutamine, 0.1 mM hypoxanthine, and 0.032 mM thymidine, 80%; heat-inactivated fetal bovine serum, 10%; heat-inactivated horse serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 2 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype
IgG1
Name of Depositor
Dana Farber Cancer Inst.
Deposited As
mouse (B cell); mouse (myeloma)
U.S. Patent Number
References

Schlossman SF, Morimoto C. Monoclonal antibody specific to a novel epitope of the LFA-1 antigen of human T lymphocytes. US Patent 5,002,869 dated Mar 26 1991

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