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SCC-PSA1
SCC-PSA1
規(guī)格:
價格:
編號:B165677
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產品名稱
SCC-PSA1
商品貨號
B165677
Organism
Mus musculus, mouse
Tissue
testes
培養(yǎng)基
Cell Type
fibroblast
Product Format
frozen
Culture Properties
adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
pluripotent teratocarcinoma
Gender
male
Strain
129/Sv
Applications
The cells can be maintained in an undifferentiated state by frequent subculture and the use of a fibroblast feeder cell layer (see ATCC CRL-1503).
In the absence of a feeder layer, the cells are encouraged to form aggregates which differentiate forming outer endodermal layers which encapsulate the aggregates.
Aggregates (embryoid bodies) can be encouraged to differentiate further by keeping them in suspension for 5 or more days.
Tested and found negative for ectromelia virus (mousepox).
Storage Conditions
liquid nitrogen vapor phase
Derivation
The SCC-PSA1 line was isolated from secondary cultures of the OT/5568 transplantable tumor.
Clinical Data
male
Comments
NOTE - NO LIVE CULTURES CAN BE SENT.
The cells can be maintained in an undifferentiated state by frequent subculture and the use of a fibroblast feeder cell layer (see ATCC CRL-1503).
In the absence of a feeder layer, the cells are encouraged to form aggregates which differentiate forming outer endodermal layers which encapsulate the aggregates.
Aggregates (embryoid bodies) can be encouraged to differentiate further by keeping them in suspension for 5 or more days.
Collect the bodies from a bacteriological dish and plate them in fresh medium in a tissue culture dish without feeder layer or gelatin.
A somewhat variable percentage will attach.
Change the medium every 2 to 3 days.
Migration of the endodermal layer should begin within 24 to 36 hours after plating.
Do not dislodge cells when changing the medium.
The SCC-PSA1 line was isolated from secondary cultures of the OT/5568 transplantable tumor.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing

Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

NOTE: Set up in advance flasks with irradiated fibroblast feeder layers (e.g., X-irradiated STO cells, ATCC 56-X™)

  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum, which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 10 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach.  Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. 
  5. To remove trypsin-EDTA solution, transfer cell suspension to a centrifuge tube and spin at approximately 125 x g for 5 to10 minutes.
  6. Discard supernatant and resuspend cells in fresh growth medium. Seed cultures with 8 x 104 cells/cm2 in flasks with feeder layer.
  7. Incubate cultures at 37°C. Subculture every 3 days to maintain in an undifferentiated proliferative state.

 

Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994

Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
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