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SK-ES-1

規格:

價格:

編號:B165701

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

SK-ES-1

商品貨號

B165701

Organism

Homo sapiens, human

Tissue

bone

培養基

Product Format

frozen

Morphology

epithelial

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

sarcoma (anaplastic osteosarcoma or Ewing's sarcoma)

Age

18 years

Gender

male

Ethnicity

Caucasian

Storage Conditions

liquid nitrogen vapor phase

Karyotype

modal number = 49; range = 44 to 51. This is a hyperdiploid human cell line with the modal chromosome number of 49 occurring in 34% of cells. However, cells with 50 chromosomes also occurred at high percentage (24%). At least 11 markers were consistently present in most cells. Among them were: der(3)t(3;12) (q21;q13); del(3) (q21); inv(1) (p36.1q21), and der(16)t(5;16) (q13;p13.3), all present in single copy per cell. At least three Y-autosome markers were detected. Single normal X chromosome was found in every cell. Normal Y chromosome was not found.

Clinical Data

18 years

Antibodies to the sarcoma lines were detected in sera of patients with sarcomas after absorption with non-sarcoma lines.

Caucasian

male

Sera from normal donors were also cytotoxic.

Antigen Expression

Antigen expression: Blood Type A; Rh+

Tumorigenic

Yes

Effects

Yes, in nude mice, forms small cell malignant tumor consistent with Ewing sarcoma

Comments

Antibodies to the sarcoma lines were detected in sera of patients with sarcomas after absorption with non-sarcoma lines. Sera from normal donors were also cytotoxic.

Complete Growth Medium

The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified , Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 15%.

Subculturing

Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended

Medium Renewal: 2 to 3 times per week

Cryopreservation

Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

STR Profile

Amelogenin: X,Y

CSF1PO: 11

D13S317: 8,9

D16S539: 11

D5S818: 12

D7S820: 10,11

THO1: 6,9.3

TPOX: 8

vWA: 14,17

Isoenzymes

AK-1, 1

ES-D, 1

G6PD, B

GLO-I, 2

PGM1, 1

PGM3, 1-2

Name of Depositor

G Trempe, LJ Old

Deposited As

Homo sapiens

Year of Origin

1971

References

Fogh J. Human tumor cells in vitro. New York: Plenum Press; 1975.

Bloom ET. Further definition by cytotoxicity tests of cell surface antigens of human sarcomas in culture. Cancer Res. 32: 960-967, 1972. PubMed: 4502173

Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871

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