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SK-MEL-1
SK-MEL-1
規格:
價格:
編號:B165705
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
SK-MEL-1
商品貨號
B165705
Organism
Homo sapiens, human
Tissue
malignant melanoma; skin; derived from metastatic site: lymphatic system
培養基
Cell Type
Melanoma
Product Format
frozen
Morphology
spherical
Culture Properties
clusters in suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
malignant melanoma
Age
29 years
Gender
male
Ethnicity
Caucasian
Applications
Antibody to this line was detected in 63% of patients with malignant melanoma and in 10% of patients with other diseases.
Electron microscopy revealed pigment granules relating both to synthesis and to phagocytosis.
Storage Conditions
liquid nitrogen vapor phase
Images
Derivation
F. Oettgen and associates isolated this line using cells obtained from the thoracic duct of a patient with widespread and rapidly progressing malignant melanoma.
Clinical Data
29 years
Caucasian
male
Antigen Expression
Antigen expression: Blood Type A; Rh+
Cellular Products
melanin
Tumorigenic
Yes
Effects
Yes, in nude mice; forms pigmented malignant melanomas; also forms tumors in the cheek pouch of cortisone treated hamsters
Comments
Electron microscopy revealed pigment granules relating both to synthesis and to phagocytosis.
Antibody to this line was detected in 63% of patients with malignant melanoma and in 10% of patients with other diseases.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Cultures can be maintained by addition or replacement of fresh medium. Establish new cultures at 1 x 105 cells/mL and maintain at 2 x 105 cells/mL.
Medium Renewal: 2 to 3 times per week

Cryopreservation
Culture medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X,Y
CSF1PO: 12,13
D13S317: 11
D16S539: 11,12
D5S818: 12,13
D7S820: 12
THO1: 6
TPOX: 11
vWA: 16,17
Isoenzymes
AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 1-2
PGM1, 1
PGM3, 1
Name of Depositor
G Trempe, LJ Old
Deposited As
Homo sapiens
References

Oettgen HF, et al. Suspension culture of a pigment-producing cell line derived from a human malignant melanoma. J. Natl. Cancer Inst. 41: 827-843, 1968. PubMed: 4879578

Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871

Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 77210034

Giraldo G, et al. Human sarcomas in culture. Foci of altered cells and a common antigen; induction of foci and antigen in human fibroblast cultures by filtrates. J. Exp. Med. 133: 454-478, 1971. PubMed: 4329447

Carey TE, et al. Cell surface antigens of human malignant melanoma: mixed hemadsorption assays for humoral immunity to cultured autologous melanoma cells. Proc. Natl. Acad. Sci. USA 73: 3278-3282, 1976. PubMed: 1067619

Geiger T, et al. Antitumor activity of a PKC-alpha antisense oligonucleotide in combination with standard chemotherapeutic agents against various human tumors transplanted into nude mice. Anticancer Drug Des. 13: 35-45, 1998. PubMed: 9474241

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