欧美自拍偷拍,欧美专区第一页,亚洲免费砖区

SK-MEL-5

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編號:B165711

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

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凍干粉

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甘油

平板

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產品名稱

SK-MEL-5

商品貨號

B165711

Organism

Homo sapiens, human

Tissue

skin: derived from metastatic axillary node.

培養基

Cell Type

Melanoma

Product Format

frozen

Morphology

stellate

Culture Properties

adherent

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

malignant melanoma

Age

24 years

Gender

female

Ethnicity

Caucasian

Applications

This cell line is a suitable transfection host. This line also serves as a source of target cells for the detection of melanoma specific antibody in patients with the disease.

Storage Conditions

liquid nitrogen vapor phase

Karyotype

modal number = 120; range = 95 to 133.
This is a hyperpentaploid human cell line with the modal chromosome number of 120, occurring in 9% of cells. The majority of cells had chromosome counts of 114-123. Among 14 marker chromosomes that were common to most cells, generally 9q+ had four copies per cell, t(1q8q) and del(5) (q34) had three copies per cell, and 14q+ had two copies per cell. Numerous others were also found in single copies, all infrequently. Three normal X chromosomes were found in most cells, N9 was absent, and N7 and N20 had seven to eight copies per cell.

Images

Derivation

This is one of a very extensive series of melanoma lines (see also ATCC HTB-71, ATCC HTB-72 and ATCC HTB-73) isolated by T. Takahashi and associates.

Clinical Data

24 years

Caucasian

female

Antigen Expression

Blood Type O; Rh+; HLA A2, A11, B40, Bw16

Tumorigenic

Yes

Effects

Yes, in nude mice; forms malignant melanoma

Comments

This is one of a very extensive series of melanoma lines (see also ATCC HTB-71, ATCC HTB-72 and ATCC HTB-73) isolated by T. Takahashi and associates.

Complete Growth Medium

The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing

Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.

Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended

Medium Renewal: 2 to 3 times per week

Cryopreservation

Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Temperature: 37°C

STR Profile

Amelogenin: X

CSF1PO: 10,13

D13S317: 10,12

D16S539: 10,12

D5S818: 11,13

D7S820: 9,12

THO1: 6,9

TPOX: 11

vWA: 14,18

Isoenzymes

AK-1, 1

ES-D, 1

G6PD, B

GLO-I, 1-2

PGM1, 1-2

PGM3, 1

Name of Depositor

T Takahashi

Deposited As

Homo sapiens

References

Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871

Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 77210034

Pollack MS, et al. HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines. J. Natl. Cancer Inst. 66: 1003-1012, 1981. PubMed: 7017212

Carey TE, et al. Cell surface antigens of human malignant melanoma: mixed hemadsorption assays for humoral immunity to cultured autologous melanoma cells. Proc. Natl. Acad. Sci. USA 73: 3278-3282, 1976. PubMed: 1067619

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