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當(dāng)前位置: 首頁 > ATCC代理 > SK-NEP-1 B165716
SK-NEP-1
SK-NEP-1
規(guī)格:
價(jià)格:
編號(hào):B165716
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
SK-NEP-1
商品貨號(hào)
B165716
Organism
Homo sapiens, human
Tissue
kidney; Derived from metastatic site: pleural effusion
培養(yǎng)基
Product Format
frozen
Morphology
rounded
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age
25 years
Gender
female
Ethnicity
Caucasian
Storage Conditions
liquid nitrogen vapor phase
Karyotype
(P12) hypotriploid to hypertriploid (+A1, +A2, +C, +D, +E, +F, +G) with abnormalities including acrocentric fragments, secondary constrictions and large subtelocentric markers
Clinical Data
25 years
Caucasian
female
Antigen Expression
Antigen expression: Blood Type A; Rh+
Tumorigenic
Yes
Effects
Yes, in nude mice; forms tumor with small cells consistent with Wilms' tumor
Comments
Ultrastructural features include few microvilli, junctional complexes, well formed Golgi, mostly smooth ER, lipid droplets, no virus particles.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified , Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 15%.
Subculturing
Cultures can be maintained by addition or replacement of fresh medium. Establish new cultures at 1 X 105 cells/mL and maintain at between 105 and 106 cells/mL.
Medium Renewal: Every 2 to 4 days
Cryopreservation
Culture medium, 95%; DMSO, 5%
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 10
D13S317: 11
D16S539: 11
D5S818: 13
D7S820: 8,10
THO1: 8,9.3
TPOX: 8,11
vWA: 15,19
Isoenzymes
AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 2
Me-2, 2
PGM1, 1-2
PGM3, 1
Name of Depositor
G Trempe, LJ Old
Deposited As
Homo sapiens
References

Fogh J. Human tumor cells in vitro. New York: Plenum Press; 1975.

Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871

Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 77210034

Fogh J. Cultivation, characterization, and identification of human tumor cells with emphasis on kidney, testis, and bladder tumors. Natl. Cancer Inst. Monogr. 49: 5-9, 1978. PubMed: 571047

Orkin SH, et al. Development of homozygosity for chromosome 11p markers in Wilms' tumour. Nature 309: 172-174, 1984. PubMed: 6325937

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