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SNU-423

規格:

價格:

編號:B165738

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

SNU-423

商品貨號

B165738

Organism

Homo sapiens, human

Tissue

liver

培養基

Product Format

frozen

Morphology

epithelial

Culture Properties

adherent

Biosafety Level

2 [Cells contain Hepatitis B virus]

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

grade III/IV, pleomorphic hepatocellular carcinoma

Age

40 years

Gender

male

Ethnicity

Asian

Karyotype

aneuploid; modal number = 79

Derivation

SNU-423 was derived in 1990 by J.-G. Park and associates from a primary hepatocellular carcinoma taken from a Korean patient who had been treated by transcatheter arterial embolization with lipoidol plus doxorubicin.

Tumor cells were initially cultured in ACL-4 medium supplemented with 5% heat-inactivated fetal bovine serum. After establishment, cultures were maintained in RPMI 1640 supplemented with 10% heat-inactivated fetal bovine serum.

Clinical Data

40 years

Asian

male

Antigen Expression

Blood Type B; Rh +

Comments

Grossly, the original tumor was single nodular with perinodular extensions.

Histologically, it was trabecular type.

The cultured cells are multinucleated.

Hepatitis B virus (HBV) DNA was detected by Southern blot hybridization.

HBV genomic RNA was not expressed.

Complete Growth Medium

RPMI 1640 medium, 90%; heat-inactivated fetal bovine serum, 10%

Subculturing

Volumes used in this protocol are for 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C

Subculture Ratio: 1:3 to 1:6

Medium Renewal: Every 2 to 3 days.

Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.

Cryopreservation

Culture medium, 95%; DMSO, 5%

STR Profile

Amelogenin: X,Y

CSF1PO: 11,12

D13S317: 10,13

D16S539: 9

D5S818: 10

D7S820: 12

THO1: 6,9

TPOX: 8

vWA: 15

Population Doubling Time

72 hrs

Name of Depositor

J Park

Deposited As

Homo sapiens

Year of Origin

1990

References

Park JG, et al. Characterization of cell lines established from human hepatocellular carcinoma. Int. J. Cancer 62: 276-282, 1995. PubMed: 7543080

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