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SNU-C2A

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價格:

編號:B165743

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

SNU-C2A

商品貨號

B165743

Organism

Homo sapiens, human

Product Format

frozen

培養基

Morphology

epithelial

Culture Properties

loosely adherent, multicell aggregates

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

colorectal carcinoma

Age

43 years

Gender

female

Ethnicity

Asian

Applications

This is one of 14 colorectal carcinoma cell lines derived by J.G. Park and associates during the years 1982 through 1985.

The cells and tumors formed from the cells produce detectable amounts of Dopa decarboxylase and the TAG-72 antigen.

Storage Conditions

liquid nitrogen vapor phase

Karyotype

modal number = 46; range = 44 to 48.
This is a pseudodiploid human cell line. The modal chromosome number is 46, occurring at 86%, with polyploidy at 16.2%. The ins(1;?)(p13;?), del(3)(p23), t(4q,?), t(8;?), del(9)(p13), del(11)(p13), 14q+ and two other marker chromosomes are common to all metaphases examined. Other altered chromosomes, if any, occur only sporadically. Normal N11 is absent. Two copies of X chromosomes are found in each cell. The Y chromosome is not found in QM stained preparations.

Images

Derivation

This is one of 14 colorectal carcinoma cell lines derived by J.G. Park and associates during the years 1982 through 1985.

The line was obtained from a third passage nude mouse xenograft of tumor fragments obtained from a patient prior to therapy.

Clinical Data

The line was obtained from a third passage nude mouse xenograft of tumor fragments obtained from a patient prior to therapy.

female

Asian

43 years

Antigen Expression

Blood type A; Rh+

Genes Expressed

carcinoembryonic antigen (CEA), 5 ng/ml per 10 exp6 cells per 10 days; TAG-72 surface antigen,Blood type A; Rh+

Cellular Products

carcinoembryonic antigen (CEA), 5 ng/ml per 10 exp6 cells per 10 days; TAG-72 surface antigen

Tumorigenic

Yes

Effects

Yes, in nude mice

(Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells)

Comments

This is one of 14 colorectal carcinoma cell lines derived by J.G. Park and associates during the years 1982 through 1985.

The line was obtained from a third passage nude mouse xenograft of tumor fragments obtained from a patient prior to therapy.

The line was initially grown in RPMI 1640 medium with 20% fetal bovine serum, but was later adapted to growth in the chemically defined medium ACL-4 (it can also be grown in medium with 10% serum).

The cells and tumors formed from the cells produce detectable amounts of Dopa decarboxylase and the TAG-72 antigen.

Complete Growth Medium

ACL-4 medium supplemented with 10% FBS
The base medium for this cell line is ATCC-formulated DMEM: F12 Medium Catalog No. 30-2006. To make the complete growth medium, add the following components to the base medium:

0.02 mg/ml insulin
0.01 mg/ml transferrin
25 nM sodium selenite
50 nM Hydrocortisone
1 ng/ml Epidermal Growth Factor (do not filter)
0.01 mM ethanolamine
0.01 mM phosphorylethanolamine
100 pM triiodothyronine
0.5% (w/v) bovine serum albumin
10 mM HEPES
0.5 mM sodium pyruvate
2mM L-glutamine(for final conc. of 4.5 mM)
10% FBS

Subculturing

Protocol: Cultures can be maintained by scraping off the attached cells and transfer along with the the floating cells into new flasks.

Medium Renewal: Two times weekly

Note: Growth is slow.

Cryopreservation

Freeze medium: Complete growth medium supplemented with an additonal 10% fetal bovine serum and 7.5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Atmosphere: air, 95%; carbon dioxide (CO₂), 5%

Temperature: 37°C

STR Profile

Amelogenin: X

CSF1PO: 11, 14

D13S317: 9, 12

D16S539: 8, 9

D5S818: 8, 9

D7S820: 13, 16

THO1: 9

TPOX: 8

vWA: 15, 19, 20

Species confirmation via CO1 assay: Human (mouse material may also be detected, likely due to this cell line being a nude mouse xenograft of tumor fragments from a human patient)

Isoenzymes

AK-1, 1

ES-D, 1

G6PD, B

GLO-I, 2

Me-2, 2

PGM1, 1

PGM3, 1

Name of Depositor

AF Gazdar

Deposited As

Homo sapiens

Passage History

The line was obtained from a third passage nude mouse xenograft of tumor fragments obtained from a patient prior to therapy.

Year of Origin

1982

References

Park JG, et al. Characteristics of cell lines established from human colorectal carcinoma. Cancer Res. 47: 6710-6718, 1987. PubMed: 3479249

Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells

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