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SNU-C2B
SNU-C2B
規(guī)格:
價(jià)格:
編號(hào):B165744
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
SNU-C2B
商品貨號(hào)
B165744
Organism
Homo sapiens, human
Tissue
cecum
培養(yǎng)基
Product Format
frozen
Morphology
epithelial
Culture Properties
loosely adherent, multicell aggregates
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
colorectal carcinoma
Age
43 years
Gender
female
Ethnicity
Asian
Applications
This is one of 14 colorectal carcinoma cell lines derived by J.G. Park and associates during the years 1982 through 1985.
The line was obtained from a fourth passage nude mouse xenograft of tumor fragments obtained from a patient prior to therapy (see also ATCC CCL-250.1 which was derived from the third nude mouse passage).
Storage Conditions
liquid nitrogen vapor phase
Karyotype
modal number = 46; range = 45 to 48.
This is a pseudodiploid human cell line. The modal chromosome number is 46, occurring at 70% with polyploidy at 16.4%. The del (3) (p23), t(4,?), t(8;?) (p22), del (9) (p13), del (11) (p13), t(1;8) (p13;p24), t(1;?) (q11;?) and 2 other marker chromosomes are common to all metaphases examined. Other structurally altered chromosomes, if any, occur only sporadically. The t(1;8) and t(1;?) are not found in CCL-250.1. Normal N8 and N11 are absent. The X chromosomes are two copies per cell. The Y chromosome is not detected by Q-banding.
Derivation
This is one of 14 colorectal carcinoma cell lines derived by J.G. Park and associates during the years 1982 through 1985.
The line was obtained from a fourth passage nude mouse xenograft of tumor fragments obtained from a patient prior to therapy (see also ATCC CCL-250.1 which was derived from the third nude mouse passage).
Clinical Data
female
Asian
43 years
Antigen Expression
Blood type A; Rh+
Receptor Expression
epidermal growth factor (EGF)
Genes Expressed
carcinoembryonic antigen (CEA), 24 ng/ml per 10 exp6 cells per 10 days,Blood type A; Rh+
Cellular Products
carcinoembryonic antigen (CEA), 24 ng/ml per 10 exp6 cells per 10 days
Tumorigenic
Yes
Effects
Yes, in nude mice
Comments

The cells have a high concentration of receptors for EGF.

Note that this line has lost both chromosomes 8 and 11 while SNU-C2A (ATCC CCL-250.1) has lost only chromosome 11.

Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 107 cells

Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing

  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
  4. Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  5. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  6. Add appropriate aliquots of the cell suspension to new culture vessels.
  7. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 11, 14
D13S317: 9, 10, 11
D16S539: 8, 9
D5S818: 8, 9
D7S820: 13, 16
THO1: 9
TPOX: 8
vWA: 15, 18, 19

Species confirmation via CO1 assay: Human (mouse material may also be detected, likely due to this cell line being a nude mouse xenograft of tumor fragments from a human patient)
Isoenzymes
AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 2
Me-2, 2
PGM1, 1
PGM3, 1
Name of Depositor
AF Gazdar
Deposited As
Homo sapiens
Passage History
The line was obtained from a fourth passage nude mouse xenograft of tumor fragments obtained from a patient prior to therapy (see also ATCC CCL-250.1 which was derived from the third nude mouse passage).
Year of Origin
1982
References

Park JG, et al. Characteristics of cell lines established from human colorectal carcinoma. Cancer Res. 47: 6710-6718, 1987. PubMed: 3479249

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