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規格:

價格:

編號:B165751

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

商品貨號

B165751

Organism

Homo sapiens, human

Tissue

pleural effusion

培養基

Cell Type

lymphoblast

Product Format

frozen

Morphology

lymphoblast

Culture Properties

suspension

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

large cell immunoblastic lymphoma

Age

11 years

Gender

male

Ethnicity

Caucasian

Storage Conditions

liquid nitrogen vapor phase

Derivation

SR is a human lymphoma cell line originated in 1983 by Walter J. Urba and Dan L. Longo.

Clinical Data

11 years

Caucasian

male

Antigen Expression

Hle-1 +; HLA DQ +; HLA DR +; CD25 +; CD19 -; CD20 -; CD21 -; CD22 -; T cell receptor (TCR) -

Comments

SR is of undetermined cellular origin because it expresses no markers unique to B, T, natural killer (NK) or monocyte-lineages.

Exposure of SR cells to protein kinase C activating phorbol esters such as PMA and PdBu do not induce growth inhibition.

SR cells have been reported to be Epstein-Barr virus genome negative.

Complete Growth Medium

The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.

Subculturing

Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 - 2 x 10⁵ viable cells/mL. Maintain cell density between 1 x 10⁵ and 1 x 10⁶ viable cells/mL.

Medium Renewal: 2 to 3 times a week.

Cryopreservation

Culture medium, 95%; DMSO, 5%

STR Profile

Amelogenin: X,Y

CSF1PO: 14,15

D13S317: 11

D16S539: 11,13

D5S818: 11

D7S820: 9,12

THO1: 6,7

TPOX: 8,11

vWA: 17,18

Name of Depositor

M Beckwith

Deposited As

Homo sapiens

References

Beckwith M, et al. Phorbol ester-induced, cell-cycle-specific, growth inhibition of human B-lymphoma cell lines. J. Natl. Cancer Inst. 82: 501-509, 1990. PubMed: 2313723

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