Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
The Src++ cell line was derived from a control mouse embryo. The cells were immortalized by infection with a retroviral vector transducing the SV40 large T antigen.
The Src++ cell line expresses endogenous wild type tyrosine kinase Src, but lacks expression of the tyrosine kinases Yes and Fyn.
Cells deficient for Src, Yes, and Fyn are available as SYF (ATCC CRL-2459).
Subcultivation Ratio: 1:4 to 1:6
Medium Renewal: Every 2 to 3 days
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
Klinghoffer RA, et al. Src family kinases are required for integrin but not PDGFR signal transduction. EMBO J. 18: 2459-2471, 1999. PubMed: 10228160
Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.
Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.
Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.
