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ST486
ST486
規格:
價格:
編號:B165757
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
ST486
商品貨號
B165757
Organism
Homo sapiens, human
Tissue
ascites
培養基
Cell Type
B lymphocyte
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
Burkitt's lymphoma
Gender
female
Storage Conditions
liquid nitrogen vapor phase
Clinical Data
female
Antigen Expression
HLA A2, A10 (Aw25), B5 (Bw52), B18
Receptor Expression
complement
Genes Expressed
IgM; kappa light chain, immunoglobulin (surface and secreted)
Cellular Products
IgM; kappa light chain
immunoglobulin (surface and secreted)
Comments
The cells are EBNA negative and are negative for Fc receptors.
Approximately 2% of the cells express commplement receptors.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 to 3 X 105 viable cells/mL. Maintain cell density between 2 X 105 and 2 X 106 viable cells/mL.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze Medium: Complete growth medium, 95%; DMSO, 5%
Storage Temperature: Liquid nitrogen vapor phase
STR Profile
Amelogenin: X
CSF1PO: 10,12
D13S317: 8,12
D16S539: 9,12
D5S818: 11,12
D7S820: 7,12
THO1: 7,9
TPOX: 8,11
vWA: 17,18
Population Doubling Time
19 to 20 hrs
Name of Depositor
IT Magrath
Deposited As
Homo sapiens
References

Benjamin D, et al. Immunoglobulin secretion by cell lines derived from African and American undifferentiated lymphomas of Burkitt's and non-Burkitt's type. J. Immunol. 129: 1336-1342, 1982. PubMed: 6286763

Magrath IT, et al. Characterization of lymphoma-derived cell lines: comparison of cell lines positive and negative for Epstein-Barr virus nuclear antigen. I. Physical, cytogenetic, and growth characteristics. J. Natl. Cancer Inst. 64: 465-476, 1980. PubMed: 6243721

Magrath IT, et al. Characterization of lymphoma-derived cell lines: comparison of cell lines positive and negative for Epstein-Barr virus nuclear antigen. II. Surface markers. J. Natl. Cancer Inst. 64: 477-483, 1980. PubMed: 6243722

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