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SV63
SV63
規(guī)格:
價(jià)格:
編號:B165766
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
SV63
商品貨號
B165766
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Product Format
frozen
培養(yǎng)基
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
Choriocarcinoma
Strain
(BALB/c X C57BL/6)F1 (B cell); BALB/c (myeloma)
Applications
The SV63 antibody reacts with an epitope present on the placental, intestinal, and germ cell forms of human alkaline phosphatase, but not on the liver/kidney/bone form of the enzyme.
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Spleen cells were fused with NS-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human choriocarcinoma tumor cell antigen
Cellular Products
immunoglobulin; monoclonal antibody; against human choriocarcinoma tumor cell antigen
Comments
Animals were immunized with the GCC-SV(c) human choriocarcinoma cell line.
Spleen cells were fused with NS-1 myeloma cells.
The SV63 antibody reacts with an epitope present on the placental, intestinal, and germ cell forms of human alkaline phosphatase, but not on the liver/kidney/bone form of the enzyme.
The antibody precipitates a glycoprotein about 68000 daltons from extracts of choriocarcinoma cells.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 10%
  • HT
  • 1 mM non-essential amino acids

Subculturing
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium.
Maintain cell density between 1 X 105 and 1 X 106 viable cells/ml.
Growth is enhanced by using a feeder layer of irradiated STO cells.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Isotype
IgG1
Name of Depositor
Sloan-Kettering Institute for Cancer Research
Deposited As
mouse (B cell); mouse (myeloma)
U.S. Patent Number
References

Rettig WJ, et al. Choriocarcinoma monoclonal antibodies and antibody panels. US Patent 4,851,332 dated Jul 25 1989

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