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When grown on a synthetic basement-like membrane, SVEC4-10 forms branching tube-like networks.
They specifically bind mouse lymphocytes in vitro.
The cells express the cell surface major histocompatibility complex class I antigen, H-2 k, and are susceptible to lysis by anti SV40 H-2 k CTL clones.
Interferon gamma (IFN-gamma) induces expression of MHC class II antigen in a time course identical to that of normal endothelial cells.
The cells express vascular cell adhesion molecule (VCAM), and stain positively for SV40 T antigen.
SVEC4-10 is the parental line for a series of endothelial cell clones (ATCC CRL-2160, ATCC CRL-2161, ATCC CRL-2162, ATCC CRL-2163, ATCC CRL-2167, ATCC CRL-2168 and ATCC CRL-2171).
O'Connell KA, Edidin M. A mouse lymphoid endothelial cell line immortalized by simian virus 40 binds lymphocytes and retains functional characteristics of normal endothelial cells. J. Immunol. 144: 521-525, 1990. PubMed: 2153170
O'Connell KA, Rudmann AA. Cloned spindle and epithelioid cells from murine Kaposi's sarcoma-like tumors are of endothelial origin. J. Invest. Dermatol. 100: 742-745, 1993. PubMed: 8496612
O'Connell K, et al. Endothelial cells transformed by SV40 T antigen cause Kaposi's sarcomalike tumors in nude mice. Am. J. Pathol. 139: 743-749, 1991. PubMed: 1928299
