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當(dāng)前位置: 首頁 > ATCC代理 > SW962 [SW 962, SW-962] B165789
SW962 [SW 962, SW-962]
SW962 [SW 962, SW-962]
規(guī)格:
價(jià)格:
編號(hào):B165789
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
SW962 [SW 962, SW-962]
商品貨號(hào)
B165789
Organism
Homo sapiens, human
Tissue
vulva; Derived from metastatic site: lymph node
培養(yǎng)基
Product Format
frozen
Morphology
mixed
Culture Properties
adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
Carcinoma
Age
64 years
Gender
female
Ethnicity
Caucasian
Karyotype
hypertriploid; modal number = 80; range = 70 to 85. The rate of higher ploidies was 4.3%., At least 15 marker chromosomes were common to most cells. These include: t(1p3p), t(1p17p), i(3q), t(8q9q), del(3)(p21), 20q+ and nine others. Of these, i(3q), t(8q9q), del(3)(p21) and a few others were generally paired., An additional segment on 20q+ is long, but its origin is unknown. The total length of 20q+ is as long as that of N1. Normal N3 was absent, and N1 and N18 had only a single copy per cell. Usually, there were three copies of normal X chromosomes.
Derivation
The SW 962 cell line was initiated by A. Leibovitz in 1975 at the Scott and White Clinic, Temple, Texas from a surgical specimen of a lymph node metastasis from carcinoma of the vulva of a 64 year old female Caucasian.
Clinical Data
The SW 962 cell line was initiated by A. Leibovitz in 1975 at the Scott and White Clinic, Temple, Texas from a surgical specimen of a lymph node metastasis from carcinoma of the vulva of a 64 year old female Caucasian.
female
Caucasian
64 years
Antigen Expression
Blood type A; Rh+
Genes Expressed
Blood type A; Rh+
Tumorigenic
Yes
Effects
Yes, in nude mice
Comments
The SW 962 cell line was initiated by A. Leibovitz in 1975 at the Scott and White Clinic, Temple, Texas from a surgical specimen of a lymph node metastasis from carcinoma of the vulva of a 64 year old female Caucasian.
A frozen ampule of the line at passage 14 was transferred to the ATCC in January, 1982.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

(Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation)


Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, rinse with fresh 0.25% trypsin solution, remove trypsin and let the culture sit at room temperature (or at 37C) until the cells detach (about 10 minutes).
Add fresh medium, aspirate and dispense into new flasks.
Subculture every 6 to 8 days.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Culture Conditions
Atmosphere: air, 100%
STR Profile
Amelogenin: X
CSF1PO: 10,11
D13S317: 11,14
D16S539: 9,11
D5S818: 12
D7S820: 10,11
THO1: 6,9.3
TPOX: 11
vWA: 15,17
Isoenzymes
AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 1
PGM1, 1-2
PGM3, 1
Name of Depositor
A Leibovitz
Passage History
A frozen ampule of the line at passage 14 was transferred to the ATCC in January, 1982.
References

Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 77210034

Wright WC, et al. Distinction of seventy-one cultured human tumor cell lines by polymorphic enzyme analysis. J. Natl. Cancer Inst. 66: 239-247, 1981. PubMed: 6935474

The cells produce tumors consistent with grade I squamous cell carcinoma.

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