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SW1463 [SW 1463, SW-1463]

規(guī)格:

價格:

編號:B165794

品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

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產(chǎn)品名稱

SW1463 [SW 1463, SW-1463]

商品貨號

B165794

Organism

Homo sapiens, human

Tissue

rectum

培養(yǎng)基

Product Format

frozen

Morphology

epithelial

Culture Properties

adherent

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

Dukes' type C, colorectal adenocarcinoma

Age

66 years

Gender

female

Ethnicity

Caucasian

Storage Conditions

liquid nitrogen vapor phase

Derivation

The line was derived from a grade II mucous producing and grade III solid tumor.

Clinical Data

66 years

Caucasian

female

Antigen Expression

blood type A; Rh +

Genes Expressed

carcinoembryonic antigen (CEA) 223 ng/10⁶ cells/10 days; keratin

Cellular Products

carcinoembryonic antigen (CEA) 223 ng/10⁶ cells/10 days; keratin

Tumorigenic

Yes

Effects

Yes, in nude mice

(Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells)

Comments

CSAp negative (CSAp-).

The cells are positive for keratin by immunoperoxidase staining.

Tumor specific nuclear matrix proteins CC-4, CC-5 and CC-6 are expressed.

Complete Growth Medium

The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

(Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation)

Subculturing

Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

Subculture just prior to confluency

Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mof complete growth medium and aspirate cells by gently pipetting.
To remove trypsin-EDTA solution, transfer cell suspension to the centrifuge tube and spin at approximately 125 x g for 5 to10 minutes.
Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels.
Incubate cultures at 37°C without CO_2.

Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended

Medium Renewal: 1 to 2 times per week

Culture Conditions

Atmosphere: Air, 100%

STR Profile

Amelogenin: X

CSF1PO: 11,12

D13S317: 12,13

D16S539: 11

D5S818: 13,14

D7S820: 9

THO1: 6,7

TPOX: 8,11

vWA: 16

Isoenzymes

ES-D, 1

G6PD, B

PEP-D, 1

PGD, A

PGM1, 1

PGM3, 1-2

Name of Depositor

A Leibovitz

Deposited As

Homo sapiens

References

Leibovitz A, et al. Classification of human colorectal adenocarcinoma cell lines. Cancer Res. 36: 4562-4569, 1976. PubMed: 1000501

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