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T1 (174 x CEM.T1)
T1 (174 x CEM.T1)
規格:
價格:
編號:B165810
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
T1 (174 x CEM.T1)
商品貨號
B165810
Organism
Homo sapiens, human
Tissue
lymphoblast
培養基
Cell Type
B lymphoblast,T lymphoblast
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
2  [Cells contain Epstein-Barr virus viral DNA sequences]

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
T1 is the parental line for the T2 (174 x CEM.T2) line (ATCC CRL-1992), and together the lines are useful for studying antigen processing and T cell recognition of class I major histocompatibility antigens.
Storage Conditions
liquid nitrogen vapor phase
Derivation
This line is a cloned hybrid between the 721.174 (variant of the LCL 721 B lymphoblastic cell line) and CEMR.3 (8-azaguanine and ouabain resistant clone of the CEM T lymphoblastic cell line).
Antigen Expression
HLA A2, Aw30, B5, Bw4, Bw6, DR7; CD7 +
Genes Expressed
HLA A2, Aw30, B5, Bw4, Bw6, DR7; CD7 +
Comments
The cells express large amounts of HLA DR7 and HLA A2.
It does not express DR3.
Complete Growth Medium
Iscove's modified Dulbecco's medium, 90%; fetal bovine serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 105 cells/mL and maintain between 1 X 105 and 1 X 106 cells/mL.
Cryopreservation
Freeze medium: complete growth medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; carbon dioxide (CO2), 5%
STR Profile
Amelogenin: X
CSF1PO: 11, 12
D13S317: 11, 12
D16S539:10,12
D5S818: 12, 14
D7S820: 10, 11, 15
THO1: 6, 9.3
TPOX: 8, 12
vWA: 14, 17
Name of Depositor
P Cresswell
Deposited As
Homo sapiens
References

Salter RD, et al. Genes regulating HLA class I antigen expression in T-B lymphoblast hybrids. Immunogenetics 21: 235-246, 1985. PubMed: 3872841

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