Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
This cell line is a suitable transfection host.
Together the T1 and T2 lines are useful for studying antigen processing and T cell recognition of class I MHC antigens.
T2 appears to have lost both copies of the CEMR.3 derived chromosome 6.
The cells do not express HLA DR and are Class II major histocompatibility (MHC) antigen negative. The cells synthesize, but do not express, HLA B5.
Salter RD, et al. Genes regulating HLA class I antigen expression in T-B lymphoblast hybrids. Immunogenetics 21: 235-246, 1985. PubMed: 3872841
Salter RD, Cresswell P. Impaired assembly and transport of HLA-A and -B antigens in a mutant TxB cell hybrid. EMBO J 5(5):943-9, 1986. PubMed: 3522223
Steinle A, Schendel DJ. HLA class I alleles of LCL 721 and 174 x CEM.T2 (T2). Tissue Antigens 44(4):268-70, 1994. PubMed: 7871529
Weinzierl AO, et al. Features of TAP-independent MHC class I ligands revealed by quantitative mass spectrometry. Eur J Immunol 38(6): 1503-10, 2008. PubMed: 18446792
