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TE 354.T

規格:

價格:

編號:B165837

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

TE 354.T

商品貨號

B165837

Organism

Homo sapiens, human

Tissue

skin

培養基

Product Format

frozen

Morphology

fibroblast

Culture Properties

adherent

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

basal cell carcinoma

Gender

female

Storage Conditions

liquid nitrogen vapor phase

Images

Clinical Data

female

Comments

The patient was sister of donor for ATCC CRL-7714.

CRL-7762 may grow very slowly. The cells may recover from cryopreservation slowly and may take as long as 10-14 days after thaw before a subculture can be performed.

Part of the NBL Collection. Unlike other cell lines in the NBL Collection, this item has been fully accessioned by ATCC and is covered by the standard warranty.

Complete Growth Medium

The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing

Note: CRL 7762 may grow very slowly. The cells may recover from cryopreservation slowly and may take as long as 10-14 days after thaw before a subculture can be performed

Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended

Medium Renewal: Every 2 to 3 days

Cryopreservation

Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Atmosphere: air, 95%; carbon dioxide (CO₂), 5%

Temperature: 37°C

STR Profile

Amelogenin: X

CSF1PO: 10,14

D13S317: 11,14